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Titolo:
Selective depression of nociceptive responses of dorsal horn neurones by SNC 80 in a perfused hindquarter preparation of adult mouse
Autore:
Cao, CQ; Hong, YG; Dray, A; Perkins, MN;
Indirizzi:
AstraZeneca R&D Montreal, Dept Pharmacol, St Laurent, PQ H4S 1Z9, Canada AstraZeneca R&D Montreal St Laurent PQ Canada H4S 1Z9 PQ H4S 1Z9, Canada
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 107, anno: 2001,
pagine: 329 - 338
SICI:
0306-4522(2001)107:2<329:SDONRO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPIOID RECEPTOR AGONISTS; RAT SPINAL-CORD; HIGH-AFFINITY; DELTA; MU; ENKEPHALINS; INHIBITIONS; STIMULI; CAT;
Keywords:
delta-opioid receptors; HS 378; naloxone; nociception; spinal cord; wind-up;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Cao, CQ AstraZeneca R&D Montreal, Dept Pharmacol, 7171 Frederick Banting, St Laurent, PQ H4S 1Z9, Canada AstraZeneca R&D Montreal 7171 Frederick Banting St Laurent PQ Canada H4S 1Z9
Citazione:
C.Q. Cao et al., "Selective depression of nociceptive responses of dorsal horn neurones by SNC 80 in a perfused hindquarter preparation of adult mouse", NEUROSCIENC, 107(2), 2001, pp. 329-338

Abstract

Detailed electrophysiological characterisation of spinal opioid receptors in the mouse has been limited due to various technical difficulties. In this study, extracellular single unit recordings were made from dorsal horn neurones in a perfused spinal cord with attached trunk-hindquarter to investigate the role of delta -opioid receptor in mediating nociceptive and non-nociceptive transmission in mouse. Noxious electrical shock, pinch and heat stimuli evoked a mean response of 20.8 +/- 2.5 (n = 10, P < 0.005), 30.1 +/-5.4 (n = 58, P < 0.005) and 40.9 +/- 6.3 (n = 29, P < 0.005) spikes per stimulus respectively. In 5 of 22 cells, repetitive noxious electrical stimuli applied to the hindpaw for 20 s produced a progressive increase in spike number, the phenomenon known as 'wind-up' and/or hyperactivity. When the selective delta -opioid receptor agonist (+)-4-[(alphaR)-alpha-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC 80) was perfused for 8-10 min, these evoked nociceptive responses were reversibly depressed. SNC 80 (2 muM) depressed the nociceptive responses evoked by electrical shock, pinch and heat by 74.0 +/- 13.7% (n = 8, P < 0.01), 66.5 +/- 16.6% (n = 10. P < 0.01) and 74.1 +/- 17.0% (n = 10, P < 0.01) respectively. The maximum depression by 5 muM SNC 80 was 92.6 +/- 6.8% (n = 3). SNC 80 at 5 muM also completely abolished the wind-up and/or hypersensitivity (n = 5), The depressant effects of SNC 80 on the nociceptive responses were completely blocked by 10 muM naloxone (n = 5) and 3 muM 17-(cyclopropylmethyl)-6,7-dehydro-4,5 alpha -epoxy- 14 beta -ethoxy-5 beta -methylindolo [2 ' ,3 ' :6 ' ,7 '] morphinan-3-ol hydrochloride (HS 378, n = 8), a novel highly selective delta -opioid receptor antagonist. Interestingly, HS 378 (3 muM) itself potentiated the background activity and evoked responses to pinch and heat by 151.8 +/- 38.4% (P < 0.05, n = 8), 34.2 +/- 6.1% (P < 0.01, n = 7) and 45.5 +/- 11.8% (P < 0.05, n = 5) respectively. In contrast, the responses of non-nociceptive dorsal horn neurotics were not inhibited by SNC 80 at a dose of up to 10 muM (n = 5). These data demonstrate that delta -opioid receptor modulate nociceptive, but not non-nociceptive, transmission in spinal dorsal horn neurones of the adult mouse. The potentiation of neuronal activity by HS 378 may reflect anautoregulatory role of the endogenous delta -opioid in nociceptive transmission in mouse. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 11/07/20 alle ore 20:50:21