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Titolo:
The PH domain of the yeast GEF Rom2p serves an essential function in vivo
Autore:
Lorberg, A; Jacoby, JJ; Schmitz, HP; Heinisch, JJ;
Indirizzi:
Univ Dusseldorf, Inst Mikrobiol, D-40225 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany Univ Hohenheim, Fachgebiet Garungstechnol 150, Inst Lebensmitteltechnol, D-70599 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70599 nol, D-70599 Stuttgart, Germany
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 3, volume: 266, anno: 2001,
pagine: 505 - 513
SICI:
1617-4615(200111)266:3<505:TPDOTY>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; GDP DISSOCIATION INHIBITOR; GTP-BINDING PROTEIN; CELL-WALL INTEGRITY; SACCHAROMYCES-CEREVISIAE; KLUYVEROMYCES-LACTIS; STRESS-RESPONSE; EXCHANGE FACTOR; BUDDING YEAST; PATHWAY;
Keywords:
signal transduction; MAP kinase; small G-protein; GDP/GTP exchange factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Heinisch, JJ Univ Dusseldorf, Inst Mikrobiol, Univ Str 1,Geb 26-12, D-40225 Dusseldorf,Germany Univ Dusseldorf Univ Str 1,Geb 26-12 Dusseldorf Germany D-40225
Citazione:
A. Lorberg et al., "The PH domain of the yeast GEF Rom2p serves an essential function in vivo", MOL GENET G, 266(3), 2001, pp. 505-513

Abstract

In a screen designed to identify new upstream components of the Pkc1p-MAP kinase signal transduction pathway that responds to cell wall damage in yeast, we identified a new mutant allele of the ROM2 gene, which encodes a GDP/GTP exchange factor that acts on Rho1p. This allele, originally termed ubk1 (upstream of Bck1p) encodes a truncated protein that lacks the putative PH domain. Complementation experiments showed that genes coding for several known components of the pathway are able to suppress the ubk1 mutation to various degrees when introduced on low- or high-copy-number vectors. Analysis of several rom2 mutants showed that mutants in which the PH domain is deleted result in a phenotype indistinguishable from that of a strain deleted for the entire gene, indicating that this domain fulfills an essential function in vivo. Furthermore, we found that the growth phenotype of rom2 mutants is highly dependent on the strain background. Surprisingly, analysis of the phosphorylation status of Mpk1p in these mutants showed an elevated level of doubly phosphorylated Mpk1 protein. indicating that the growth defectof rom2 mutants is not due to an inability to activate the MAP kinase module, but rather to lack of a function of the Rom2 protein that has yet to beidentified precisely.

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Documento generato il 01/04/20 alle ore 11:40:39