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Titolo:
Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) asrevealed by AFLP analysis and a new set of microsatellite markers
Autore:
David, L; Rajasekaran, P; Fang, J; Hillel, J; Lavi, U;
Indirizzi:
Agr Res Org, Volcani Ctr, Inst Hort, IL-50250 Bet Dagan, Israel Agr Res Org Bet Dagan Israel IL-50250 t Hort, IL-50250 Bet Dagan, Israel Hebrew Univ Jerusalem, Fac Agr Food & Environm Sci, Dept Field Crops & Genet, IL-76100 Rehovot, Israel Hebrew Univ Jerusalem Rehovot Israel IL-76100 , IL-76100 Rehovot, Israel Scottish Crop Res Inst, Unit Appl Genet, Dundee DD2 5DA, Scotland ScottishCrop Res Inst Dundee Scotland DD2 5DA Dundee DD2 5DA, Scotland Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China Nanjing Agr Univ Nanjing Peoples R China 210095 210095, Peoples R China
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 3, volume: 266, anno: 2001,
pagine: 353 - 362
SICI:
1617-4615(200111)266:3<353:PIOACC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-LINKAGE MAP; INHERITANCE; LOCI; CONSERVATION; TILAPIA; CATTLE; GENOME; SALMON;
Keywords:
Koi carp; grass carp; microsatellite; AFLP; genetic distance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Lavi, U Agr Res Org, Volcani Ctr, Inst Hort, POB 6, IL-50250 Bet Dagan, Israel Agr Res Org POB 6 Bet Dagan Israel IL-50250 250 Bet Dagan, Israel
Citazione:
L. David et al., "Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) asrevealed by AFLP analysis and a new set of microsatellite markers", MOL GENET G, 266(3), 2001, pp. 353-362

Abstract

Forty-seven new microsatellite markers were generated and applied, together with the AFLP (Amplified Fragment Length Polymorphism) technique using two different enzyme combinations, to the genetic analysis of two carp species, Cyprinus carpio L. and Ctenopharyngodon idella. The extent of polymorphism and the genetic relationships between nine carp populations were studied. The incidence of microsatellites containing CA and CT motifs was estimated to be one every 17.4 and one every 126.3 kb, respectively, and their average allele numbers were four and five, respectively. Across populations, the average proportion of individuals that were heterozygous for microsatellite markers was 44.2% and the average allele number was 4.02. The EcoRI/TaqIcombination generated more analyzable AFLP bands than the EcoRI/MseI pair,making the former preferable for the analysis of carp populations. The proportion of polymorphic AFLP bands within populations ranged from 6.7% in grass carp to 59.9% in Kohaku strain (Koi) of the ornamental carp. The fixation index (F-ST) for microsatellites in these populations was estimated to be 0.37, and for AFLP markers the value was 0.39. Genetic distance matrices derived from microsatellites. and from two AFLP analyses were positively correlated. Grass carp showed fewer AFLP bands than other populations and wasgenotyped by only half of the microsatellite markers. These findings agreewith genetic distance estimates in suggesting that the grass carp is phylogenetically quite remote from all the other populations examined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:54:37