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Titolo:
The N-terminal domain of the t-SNARE Vam3p coordinates priming and dockingin yeast vacuole fusion
Autore:
Laage, R; Ungermann, C;
Indirizzi:
Univ Heidelberg, Zentrum Biochem, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 11, volume: 12, anno: 2001,
pagine: 3375 - 3385
SICI:
1059-1524(200111)12:11<3375:TNDOTT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-FUSION; PROTEIN-TRANSPORT; SACCHAROMYCES-CEREVISIAE; NEUROTRANSMITTER RELEASE; 3-DIMENSIONAL STRUCTURE; COMPLEX-FORMATION; HOMOTYPIC FUSION; SYNTAXIN 1A; ALPHA-SNAP; LATE STEP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Ungermann, C Univ Heidelberg, Zentrum Biochem, Neuenheimer Feld 230, D-69120 Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 230 Heidelberg Germany D-69120
Citazione:
R. Laage e C. Ungermann, "The N-terminal domain of the t-SNARE Vam3p coordinates priming and dockingin yeast vacuole fusion", MOL BIOL CE, 12(11), 2001, pp. 3375-3385

Abstract

Homotypic fusion of yeast vacuoles requires a regulated sequence of events. During priming, Sec18p disassembles cis-SNARE complexes. The HOPS complex, which is initially associated with the cis-SNARE complex, then mediates tethering. Finally, SNAREs assemble into trans-complexes before the membranes fuse. The t-SNARE of the vacuole, Vam3p, plays a central role in the coordination of these processes. We deleted the N-terminal region of Vam3p to analyze the role of this domain in membrane fusion. The truncated protein (Vam3 DeltaN) is sorted normally to the vacuole and is functional, because the vacuolar morphology is unaltered in this strain. However, in vitro vacuole fusion is strongly reduced due to the following reasons: Assembly, as well as disassembly of the cis-SNARE complex is more efficient on Vam3 DeltaN vacuoles; however, the HOPS complex is not associated well with the Vam3 DeltaN cis-complex. Thus, primed SNAREs from Vam3 DeltaN vacuoles cannot participate efficiently in the reaction because trans-SNARE pairing is substantially reduced. We conclude that the N-terminus of Vam3p is required for coordination of priming and docking during homotypic vacuole fusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:44:19