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Titolo:
Prolactin, interleukin-2 and FGF-2 stimulate expression, nuclear distribution and DNA-binding of rat homolog of pombe Cdc5 in Nb2 T lymphoma cells
Autore:
Johnson, LM; Too, CKL;
Indirizzi:
Dalhousie Univ, Fac Med, Dept Biochem & Mol Biol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 iol, Halifax, NS B3H 4H7, Canada Dalhousie Univ, Fac Med, Dept Obstet & Gynecol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 col, Halifax, NS B3H 4H7, Canada
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 184, anno: 2001,
pagine: 151 - 161
SICI:
0303-7207(20011126)184:1-2<151:PIAFSE>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE NA+/H+ EXCHANGE; SCHIZOSACCHAROMYCES-POMBE; GROWTH-HORMONE; MESSENGER-RNA; MYB; PROTEIN; IDENTIFICATION; 12-O-TETRADECANOYL-PHORBOL-13-ACETATE; COMPLEX;
Keywords:
prolactin; IL-2; FGF-2; Cdc5; rat Nb2 lymphoma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Too, CKL Dalhousie Univ, Fac Med, Dept Biochem & Mol Biol, Sir Charles Tupper Med Bldg, Halifax, NS B3H 4H7, Canada Dalhousie Univ Sir Charles TupperMed Bldg Halifax NS Canada B3H 4H7
Citazione:
L.M. Johnson e C.K.L. Too, "Prolactin, interleukin-2 and FGF-2 stimulate expression, nuclear distribution and DNA-binding of rat homolog of pombe Cdc5 in Nb2 T lymphoma cells", MOL C ENDOC, 184(1-2), 2001, pp. 151-161

Abstract

Pombe and human Cdc5 have been implicated in G2/M progression, but recently Cdc5 was identified as a component of a multiprotein complex essential for pre-mRNA splicing. We have previously isolated a prolactin (PRL)-inducible partial CDNA (1907 bp) encoding rat Cdc5. In the present study, the full length rCdc5 sequence (2847 bp) was obtained by 5'-RACE and cytokine regulation of Cdc5 expression was examined. PRL and interleukin-2 (IL2) act as mitogens in Nb2 T-lymphoma cells. Fibroblast growth factor (FGF-2) is not mitogenic in Nb2 cells but inhibits apoptosis of PRL-deprived cells. This study showed that PRL, IL-2 and FGF-2 rapidly increased Nb2 Cdc5 expression (3.4 kb mRNA) to reach 2-3-fold above controls at 4 h, and Cdc5 mRNA levels remained elevated at 24 h. There was a corresponding 2-3-fold increase in Cdc5 protein (105 kDa) levels at 24 h. Immunoblotting and fluorescent confocalmicroscopy showed predominant nuclear/perinuclear Cdc5 in quiescent Nb2 cells. PRL or FGF-2 treatment transiently increased nuclear Cdc5-specific immunofluorescence at 4 It but IL-2 gave maximal nuclear accumulation of Cdc5 at 24 h. The deduced rCdc5 protein has similar to 98% amino acid identity with human Cdc5. Like other Cdc5 family members. the N-terminus of rCdc5 contains two repeats of a DNA-binding domain found in a-, b- and c-Myb. Gel shift assays using P-32-labeled Myb consensus oligonucleotides revealed two Myb-specific DNA-protein complexes in Nb2 nuclear extracts, Formation of both complexes was increased by PRL or FGF-2 at 1-5 and at 20 h and was partially inhibited by anti-Myb or anti-Cdc5 antibodies. In summary, rapid activation of Cdc5 in response to mitogenic and non-mitogenic stimuli suggests a complex role for Cdc5 in cellular regulation and this may not be restrictedto mitotic entry or G2/M progression as previously supposed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:04:00