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Titolo:
Microsatellite genotyping of individual abalone larvae: Parentage assignment in aquaculture
Autore:
Selvamani, MJP; Degnan, SM; Degnan, BN;
Indirizzi:
Univ Queensland, Dept Zool & Entomol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 5, volume: 3, anno: 2001,
pagine: 478 - 485
SICI:
1436-2228(200109/10)3:5<478:MGOIAL>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HALIOTIS-ASININA LINNE; TROPICAL ABALONE; STOCK ENHANCEMENT; BLACKLIP ABALONE; RAINBOW-TROUT; EAR ABALONE; HERON-REEF; LOCI; GROWTH; DNA;
Keywords:
abalone; aquaculture; fertilization; Haliotis asinina; selective breeding; sperm competition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Degnan, BN Univ Queensland, Dept Zool & Entomol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 ld 4072, Australia
Citazione:
M.J.P. Selvamani et al., "Microsatellite genotyping of individual abalone larvae: Parentage assignment in aquaculture", MAR BIOTEC, 3(5), 2001, pp. 478-485

Abstract

In aquaculture, microsatellite DNA markers are used to genotype parental broodstock, to assess fertilization Success, and to maintain pedigree information for selective breeding. In this study we genotyped individual Haliotis asinina larvae by analyzing a suit of polymorphic microsatellite loci. Atleast 10 loci can be analyzed front a single abalone veliger larva. We assayed 5 polymorphic loci to identify the parents of individual larvae produced in 3 separate crosses. In all cases, the parents of an individual veliger could be determined from as few as 3 loci. The microsatellite analysis revealed that, in each of our crosses, a single male fathered most of the veligers, despite efforts to normalize the amount of sperm contributed by competing males. These observations suggest that highly controlled breeding practices may be required to ensure that the genetic diversity of an abalone population produced for aquaculture is maintained at the level of diversity of the original broodstock.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 14:38:13