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Titolo:
Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer)
Autore:
Ma, P; Sivaloganathan, B; Reddy, PK; Chan, WK; Lam, TJ;
Indirizzi:
Natl Univ Singapore, Dept Sci Biol, Singapore 119260, Singapore Natl Univ Singapore Singapore Singapore 119260 ngapore 119260, Singapore Natl Univ Singapore, Trop Marine Sci Inst, Singapore 119223, Singapore Natl Univ Singapore Singapore Singapore 119223 ngapore 119223, Singapore
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 5, volume: 3, anno: 2001,
pagine: 463 - 469
SICI:
1436-2228(200109/10)3:5<463:OOAGEI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
DICENTRARCHUS-LABRAX LARVAE; DIGESTIVE ENZYMES; PCR; QUANTITATION; QUANTIFICATION; AMPLIFICATION; TISSUES; ACIDS; EGGS;
Keywords:
alpha-amylase; sea bass larvae; real-time PCR; mRNA expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Lam, TJ Natl Univ Singapore, Dept Sci Biol, 10 Kent Ridge Crescent, Singapore 119260, Singapore Natl Univ Singapore 10 Kent Ridge Crescent SingaporeSingapore 119260
Citazione:
P. Ma et al., "Ontogeny of alpha-amylase gene expression in sea bass larvae (Lates calcarifer)", MAR BIOTEC, 3(5), 2001, pp. 463-469

Abstract

The enzymatic activities of alpha -amylase and its corresponding messengerRNA levels in developing sea bass (Lates calcarifer) larvae were studied from hatching until 27 days post hatching (dph). An increasing activity of amylase enzyme was measured until 5 dph, and the activity gradually decreased thereafter and reached a constant level by 12 dph. To achieve a better understanding of the molecular mechanisms underlying amylase expression, we have cloned and sequenced a 318-bp fragment of alpha -amylase complementary DNA. Based on this sequence, a real-time reverse transcriptase polymerase chain reaction technique to monitor the changes in the mRNA levels in the larvae was developed. A correlation between enzymatic activity and mRNA levelof alpha -amylase could be demonstrated during the early development of sea bass larvae. This suggests that the changes in alpha -amylase are controlled at least at the transcriptional level during early larval development of sea bass.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:35:23