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Titolo:
Expression of aryl hydrocarbon receptor nuclear translocator (ARNT) isoforms in juvenile and adult rainbow trout tissues
Autore:
Sojka, KM; Pollenz, RS;
Indirizzi:
Univ S Florida, Dept Biol, Tampa, FL 33620 USA Univ S Florida Tampa FL USA 33620 Florida, Dept Biol, Tampa, FL 33620 USA Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA MedUniv S Carolina Charleston SC USA 29425 iol, Charleston, SC 29425 USA
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 5, volume: 3, anno: 2001,
pagine: 416 - 427
SICI:
1436-2228(200109/10)3:5<416:EOAHRN>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED SIGNAL-TRANSDUCTION; HYPOXIA-INDUCIBLE FACTOR-1; AH RECEPTOR; ONCORHYNCHUS-MYKISS; DISTINCT FUNCTIONS; DEVELOPING MOUSE; MESSENGER-RNAS; PROTEIN; ZEBRAFISH; CELLS;
Keywords:
ARNT; rainbow trout; dominant negative; signal transduction; isoforms;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Pollenz, RS Univ S Florida, Dept Biol, 4202 E Fowler Ave,SCA 110, Tampa, FL 33620 USA Univ S Florida 4202 E Fowler Ave,SCA 110 Tampa FL USA 33620 SA
Citazione:
K.M. Sojka e R.S. Pollenz, "Expression of aryl hydrocarbon receptor nuclear translocator (ARNT) isoforms in juvenile and adult rainbow trout tissues", MAR BIOTEC, 3(5), 2001, pp. 416-427

Abstract

Whether and where rainbow trout aryl hydrocarbon receptor nuclear translocator (rtARNT) isoforms are expressed in juvenile and adult tissues of rainbow trout is unknown. Using reverse transcriptase polymerase chain reaction (RT-PCR), expression of the rtARNT(b) isoform messenger RNA was identified in day 19 and 23 embryos, in day 27, 35, 39, and 42 sac fry, and in all adult tissues investigated. The rtARNT(a) isoform mRNA was expressed in all juvenile trout except day 42 sac fry and in all adult tissues except skeletalMuscle. Western Not analysis and immunohistochemistry demonstrated that the rtARNT(a) protein was present in all Juvenile trout and adult tissues investigated, except skeletal muscle, and was primarily localized to the nucleus. In contrast, rtARNT(a) protein was not detected at any developmental stage but was expressed in the adult gill. These results imply that rtARNT(b)is invoked in signaling events at many developmental stages, while the functionality of the dominant negative rtARNT(a) is restricted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:38:30