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Titolo:
MR microscopy of multicomponent diffusion in single neurons
Autore:
Grant, SC; Buckley, DL; Gibb, S; Webb, AG; Blackband, SJ;
Indirizzi:
Univ Florida, Dept Neurosci, McKnight Brain Inst, Gainesville, FL 32601 USA Univ Florida Gainesville FL USA 32601 ain Inst, Gainesville, FL 32601 USA Univ Illinois, Dept Bioengn, Chicago, IL USA Univ Illinois Chicago IL USA niv Illinois, Dept Bioengn, Chicago, IL USA Natl High Magnet Field Lab, Tallahassee, FL USA Natl High Magnet Field Lab Tallahassee FL USA d Lab, Tallahassee, FL USA Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA Univ IllinoisUrbana IL USA 61801 Elect & Comp Engn, Urbana, IL 61801 USA
Titolo Testata:
MAGNETIC RESONANCE IN MEDICINE
fascicolo: 6, volume: 46, anno: 2001,
pagine: 1107 - 1112
SICI:
0740-3194(200112)46:6<1107:MMOMDI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL SLICE; NMR-SPECTROSCOPY; WATER DIFFUSION; SELF-DIFFUSION; BRAIN-TISSUE; RELAXATION; ATTENUATION; SYSTEMS; VOLUME; MODEL;
Keywords:
diffusion; compartmentalization; microcoils; microscopy; neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Grant, SC Univ Florida, Dept Neurosci, McKnight Brain Inst, POB 100244, Gainesville,FL 32601 USA Univ Florida POB 100244 Gainesville FL USA 32601 le,FL 32601 USA
Citazione:
S.C. Grant et al., "MR microscopy of multicomponent diffusion in single neurons", MAGN RES M, 46(6), 2001, pp. 1107-1112

Abstract

This study examines multicomponent diffusion in isolated single neurons and discusses the implications of the results for macroscopic water diffusionin tissues. L7 Aplysia neurons were isolated and analyzed using a 600 MHz Bruker wide-bore instrument with a magnetic susceptibility-matched radiofrequency microcoil. Using a biexponential fit, the apparent diffusion coefficients (ADCs) from the cytoplasm (with relative fraction) were 0.48 +/- 0.14x 10(-3) mm(2)S(-1) (61 +/- 11%) for the fast component, and 0.034 +/- 0.017 x 10(-3) mm(2)s(-1) (32 +/- 11 %) for the slow component (N = 10). Diffusion in the nucleus appears to be primarily monoexponential, but with biexponential analysis it yields 1.31 +/- 0.32 x 10(-3) mm(2)s(-1) (89 +/- 6%) for the fast component and 0.057 +/- 0.073 x 10(-3) mm(2)s(-1) (11 +/- 6%) for the slow (N = 5). The slow component in the nucleus may be explained by cytoplasmic volume averaging. These data demonstrate that water diffusion in the cytoplasm of isolated single Aplysia neurons supports a multiexponential model. The ADCs are consistent with previous measurements in the cytoplasm of single neurons and with the slow ADC measurement in perfused brain slices. These distributions may explain the multiple compartments observed in tissues, greatly aiding the development of quantitative models of MRI in whole tissues. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:08:05