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Titolo:
Glucose and prednisolone alter basic fibroblast growth factor expression in peritoneal mesothelial cells and fibroblasts
Autore:
Ogata, S; Yorioka, N; Kohno, N;
Indirizzi:
Hiroshima Univ, Sch Med, Dept Internal Med 2, Minami Ku, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 nami Ku, Hiroshima 7348551, Japan
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 12, volume: 12, anno: 2001,
pagine: 2787 - 2796
SICI:
1046-6673(200112)12:12<2787:GAPABF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOCORTICOID RECEPTOR; ENDOTHELIAL-CELLS; DIALYSIS PATIENTS; IN-VIVO; PROLIFERATION; MEMBRANE; LOCALIZATION; MACROPHAGES; VASCULATURE; MECHANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Yorioka, N Hiroshima Univ, Sch Med, Dept Internal Med 2, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348551, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348551 551, Japan
Citazione:
S. Ogata et al., "Glucose and prednisolone alter basic fibroblast growth factor expression in peritoneal mesothelial cells and fibroblasts", J AM S NEPH, 12(12), 2001, pp. 2787-2796

Abstract

The mechanism of peritoneal fibrosis in patients on continuous ambulatory peritoneal dialysis is poorly understood. The production of basic fibroblast growth factor (bFGF) by human peritoneal mesothelial cells cultured in high glucose medium was investigated, and the behavior of peritoneal fibroblasts, as well as the inhibitory effect of prednisolone, was assessed. Reverse transcriptase-PCR and immunocytochemistry showed the expression of glucocorticoid receptors in mesothelial cells. The semiquantitative reverse transcriptase-PCR showed that high glucose medium (4.0%) increased bFGF mRNA by 2.5-fold relative to control medium (0.1% glucose), with 83% suppression ofthe increase by 1 muM prednisolone. The bFGF protein level in culture supernatant was also increased by 1.5-fold in high glucose medium, with this change showing 45% suppression by 1 muM prednisolone. These effects of prednisolone were prevented by a glucocorticoid receptor antagonist (RU486) in a concentration-dependent manner. The proliferation of peritoneal fibroblastswas increased 1.9-fold by the supernatant of mesothelial cells cultured inhigh glucose medium, with 85% suppression by 1 muM prednisolone and suppression to 16% below basal proliferation by an anti-bFGF neutralizing antibody (10 mug/ml), whereas proliferation showed a concentration-dependent increase on addition of an anti-transforming growth factor beta-neutralizing antibody. Recombinant bFGF (50 to 1000 pg/ml) likewise caused a concentration-dependent increase of peritoneal fibroblast proliferation and fibronectin release by these cells was also increased (at 50 to 5000 pg/ml). These results suggest the potential importance of bFGF for initiation of peritoneal fibrosis and the possible efficacy of glucocorticoids for preventing such fibrosis in patients receiving peritoneal dialysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 02:32:23