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Titolo:
Hepatocyte growth factor alters renal epithelial cell susceptibility to uropathogenic Escherichia coli
Autore:
Wu, JH; Billings, BJ; Balkovetz, DF;
Indirizzi:
Birmingham Vet Affairs Med Ctr, Birmingham, AL USA Birmingham Vet Affairs Med Ctr Birmingham AL USA Ctr, Birmingham, AL USA Univ Alabama, Dept Med, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 , Dept Med, Birmingham, AL 35294 USA Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Cell Biol, Birmingham, AL 35294 USA Univ Alabama, Dept Surg, Birmingham, AL 35294 USA Univ Alabama BirminghamAL USA 35294 Dept Surg, Birmingham, AL 35294 USA
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 12, volume: 12, anno: 2001,
pagine: 2543 - 2553
SICI:
1046-6673(200112)12:12<2543:HGFARE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
E-CADHERIN; POLARITY; KIDNEY; MONOLAYERS; IDENTIFICATION; CYTOTOXICITY; GRANULOCYTES; REGENERATION; OBSTRUCTION; POLYPEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Balkovetz, DF 668 LHRB,1530 3rd Ave S, Birmingham, AL 35294 USA 668 LHRB,1530 3rd Ave S Birmingham AL USA 35294 L 35294 USA
Citazione:
J.H. Wu et al., "Hepatocyte growth factor alters renal epithelial cell susceptibility to uropathogenic Escherichia coli", J AM S NEPH, 12(12), 2001, pp. 2543-2553

Abstract

The urinary tract is frequently the source of Escherichia coli bacteremia. Bacteria from the urinary tract must cross an epithelial layer to enter the bloodstream. Hepatocyte growth factor (HGF) alters the polarity of Madin-Darby canine kidney (MDCK) epithelial cells. The role of cell polarity in determining renal epithelial resistance to Escherichia coli invasion is not well known. A model of polarized and HGF-treated MDCK epithelial cells grown on filters was used to study the role of epithelial cell polarity during the interaction of nonvirulent (XL1-Blue) and uropathogenic (J96) strains of Escherichia coli with renal epithelium. Basolateral. exposure of MDCK cells to J96, but not XL1-Blue, resulted in loss of transepithelial resistance(TER), which was due to epithelial cytotoxicity and not degradation of epithelial junctional proteins by bacterial proteases. Apical exposure to bothJ96 and XL1-Blue did not alter TER. Pretreatment of polarized MDCK cell monolayers with HGF renders the cells sensitive to loss of TER and cytotoxicity by apical exposure to J96. Analysis by confocal microscopy demonstrated that HGF treatment of MDCK cell monolayers also greatly enhances adherence of J96 to the apical surface of the cell monolayer. These data demonstrate that the basolateral surface of polarized epithelia is more susceptible to J96 cytotoxicity. The data also support the hypothesis that processes that alter epithelial cell polarity increase sensitivity of epithelia to bacterial injury and adherence from the apical compartment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 01:55:30