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Titolo:
Spectrofluorimetric determination of vigabatrin and gabapentin in urine and dosage forms through derivatization with fluorescamine
Autore:
Belal, F; Abdine, H; Al-Majed, A; Khalil, NY;
Indirizzi:
King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, Riyadh 11451, Saudi Arabia King Saud Univ Riyadh Saudi Arabia 11451 hem, Riyadh 11451, Saudi Arabia
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 1-2, volume: 27, anno: 2002,
pagine: 253 - 260
SICI:
0731-7085(20020101)27:1-2<253:SDOVAG>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; GAMMA-AMINOBUTYRIC ACID; SOLID-PHASE EXTRACTION; MASS-SPECTROMETRY; FLUORESCENCE DETECTION; HUMAN SERUM; PLASMA; ENANTIOMERS; FLUIDS;
Keywords:
vigabatrin; gabapentin; fluorescamine; dosage forms; urine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Belal, F King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, POB 2457, Riyadh 11451, Saudi Arabia King Saud Univ POB 2457 Riyadh Saudi Arabia 11451 , Saudi Arabia
Citazione:
F. Belal et al., "Spectrofluorimetric determination of vigabatrin and gabapentin in urine and dosage forms through derivatization with fluorescamine", J PHARM B, 27(1-2), 2002, pp. 253-260

Abstract

A stability-indicating, sensitive, simple and selective spectrofluorimetric method was developed for the determination of vigabatrin (VG) and gabapentin (GB). The method is based on the reaction between the two drugs and fluorescamine in borate buffer of pH 8.2 to give highly fluorescent derivatives that are measured at 472 nm using an excitation wavelength of 390 nn for both drugs. The optimum conditions were ascertained and the method was applied for the determination of VG and GB over the concentration range of 0.20-4.00 and 0.1-1.0 mug/ml, respectively with detection limits of 0.05 mug/ml(2.9 x 10(-7) M) and 0.06 mug/ml (2.3 x 10(-7) M) for VG and GB, respectively. The suggested method was applied, without any interference from the excipients, to the determination of the two drugs in their pharmaceutical formulations. Furthermore, the method was extended to the in-vitro determination of both drugs in spiked human urine. Interference from endogenous amino acids could be eliminated through selective complexation with copper acetate, the % recovery (n = 4) is 98.0 +/- 7.05. Co-administered drugs such as lamotrigine, phenobarbitone, valproic acid, clopazam, carbamazepine, clonazepam and cimitidine did not interfere with the assay. The method is also stability-indicating; as the degradation product of vigabatrin: 5-vinylpyrrolidin-2-one, produced no interference with its analysis. (C) 2002 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:27:12