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Titolo:
The effects of interleukin-10 depletion in vivo on the immune response to Porphyromonas gingivalis in a murine model
Autore:
Herminajeng, E; Sosroseno, W; Bird, PS; Seymour, GJ;
Indirizzi:
Univ Sains Malaysia, Sch Dent Sci, Kota Baharu 16150, Kelantan, Malaysia Univ Sains Malaysia Kota Baharu Kelantan Malaysia 16150 elantan, Malaysia Gadjah Mada Univ, Dept Oral Med, Fac Dent, Yogyakarta, India Gadjah Mada Univ Yogyakarta India Oral Med, Fac Dent, Yogyakarta, India Gadjah Mada Univ, Dept Oral Biol, Fac Dent, Yogyakarta, India Gadjah Mada Univ Yogyakarta India ral Biol, Fac Dent, Yogyakarta, India Univ Queensland, Sch Dent, St Lucia, Qld 4067, Australia Univ Queensland St Lucia Qld Australia 4067 St Lucia, Qld 4067, Australia
Titolo Testata:
JOURNAL OF PERIODONTOLOGY
fascicolo: 11, volume: 72, anno: 2001,
pagine: 1527 - 1534
SICI:
0022-3492(200111)72:11<1527:TEOIDI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY PERIODONTAL-DISEASE; ANTICOLLAGEN ANTIBODY-PRODUCTION; BACTEROIDES-GINGIVALIS; T-CELLS; CYTOKINE PROFILES; MONONUCLEAR-CELLS; EXPRESSION; MICE; HYPERSENSITIVITY; LYMPHOCYTES;
Keywords:
Porphyromonas gingivalis; lipopolysaccharides; interleukin-10; IFN-gamma; antibodies; animal studies; disease models; animal; immune response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Sosroseno, W Univ Sains Malaysia, Sch Dent Sci, Kota Baharu 16150, Kelantan, Malaysia Univ Sains Malaysia Kota Baharu Kelantan Malaysia 16150 ysia
Citazione:
E. Herminajeng et al., "The effects of interleukin-10 depletion in vivo on the immune response to Porphyromonas gingivalis in a murine model", J PERIODONT, 72(11), 2001, pp. 1527-1534

Abstract

Background: The immune response to Porphyromonas gingivalis in the mouse abscess model is known to be dependent upon CD4 T-cell activation and the regulatory role of cytokines. The role of interleukin-10 (IL-10) in this mouse model was examined in vivo. Methods: One-week-old, female BALB/c mice were divided into 4 groups. Groups 1 and 2 were given intraperitoneal (ip) injections of phosphate bufferedsaline (PBS) weekly for 5 weeks. Group 3 was given an ip injection of rat immunoglobulin. Group 4 was injected with rat anti-IL-10 antibodies. At week 6, group 1 was sham-immunized with PBS, and groups 2, 3, and 4 were injected with P gingivalis lipopolysaccharide (Pg-LPS) weekly for 2 weeks. One week after the final immunization, delayed-type hypersensitivity (DTH) was assessed by footpad swelling to Pg-LPS. The level of serum antibodies to Pg-LPS and IFN-gamma (IFN-gamma) was determined by enzyme-linked immunosorbentassay. Dorsal abscess formation induced by the injection of viable P gingivalis was examined daily for 30 days. Results: The footpad swelling of the anti-IL-10-treated group (group 4) was significantly higher than that of groups 1 to 3. Similarly, the serum IFN-gamma level in group 4 was much higher than that of the other experimentalgroups. There was no significant difference in serum IgG antibodies to Pg-LPS in any of the experimental groups. However, the level of IgM antibodiesin group 4 mice was significantly lower than that in groups 2 and 3. In addition, serum IgG1 was suppressed in group 4 mice, while IgG2a antibodies were raised. However, there was no difference observed between the levels ofIgG2b and IgG3 antibodies in any group of mice. The lesions in sham-immunized mice (group 1) persisted for 30 days, and those in group 2 and 3 were undetected by day 18 and 20, respectively. In sharp contrast, lesions in group 4 had healed completely by day 13. Conclusions: This study has shown that IL-10 depletion in vivo in P gingivalis LPS-induced immune response in mice led to an elevated DTH response, an increase in serum IFN-gamma levels, and raised levels of IgG and IgG2a antibodies. Treatment with anti-IL-10 antibodies resulted in suppressed IgG Iand IgM responses and a more rapid healing of abscesses than in non-IL-10-depleted mice. These results suggest that IL-10 depletion in Pg-LPS-inducedimmune response in mice may lead to a Th1-like immune response and providestrong protection against a subsequent challenge with live P gingivalis inan abscess model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:27:04