Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evidence that orexin-containing neurones provide direct input to gonadotropin-releasing hormone neurones in the ovine hypothalamus
Autore:
Iqbal, J; Pompolo, S; Sakurai, T; Clarke, IJ;
Indirizzi:
Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia Prince Henrys Inst Med Res Clayton Vic Australia 3168 Vic 3168, Australia Univ Tsukuba, Inst Basic Med Sci, Dept Pharmacol, Tsukuba, Ibaraki 305, Japan Univ Tsukuba Tsukuba Ibaraki Japan 305 macol, Tsukuba, Ibaraki 305, Japan
Titolo Testata:
JOURNAL OF NEUROENDOCRINOLOGY
fascicolo: 12, volume: 13, anno: 2001,
pagine: 1033 - 1041
SICI:
0953-8194(200112)13:12<1033:ETONPD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; NEUROPEPTIDE-Y NPY; LUTEINIZING-HORMONE; OVARIECTOMIZED EWES; HYPOCRETIN OREXIN; FEEDING-BEHAVIOR; ARCUATE NUCLEUS; GROWTH-HORMONE; PREOPTIC AREA; LH-SECRETION;
Keywords:
leptin; food intake; neuroendocrine regulation; preoptic area; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Clarke, IJ Prince Henrys Inst Med Res, POB 5152, Clayton, Vic 3168, Australia Prince Henrys Inst Med Res POB 5152 Clayton Vic Australia 3168
Citazione:
J. Iqbal et al., "Evidence that orexin-containing neurones provide direct input to gonadotropin-releasing hormone neurones in the ovine hypothalamus", J NEUROENDO, 13(12), 2001, pp. 1033-1041

Abstract

Orexins A and B (ORX) have been added recently to the growing list of neuropeptides implicated in feeding and drinking behaviour as well as neuroendocrine function, In the present study, we have used single and dual labelling immunohistochemistry and a rabbit polyclonal anti-orexin-A antibody, which recognizes both ORX A and B, to examine ORX pathways in the sheep hypothalamus. ORX immunoreactive cells were distributed in the dorsomedial hypothalamic nucleus, lateral hypothalamic area, zona incerta and perifornical area; a few cells were also observed in the anterior hypothalamic area. in contrast to distribution in the rat brain, most of the ORX immunoreactive cells are localized to the dorsomedial hypothalamic nucleus and perifornical area; scattered cells are found in lateral hypothalamic area. ORX immunoreactive fibres were widely distributed throughout the hypothalamus and preopticarea with dense innervation of the medial preoptic area and bed nucleus ofstria terminalis. Dual labelling demonstrated widespread expression of thelong form of the leptin receptor within all ORX cells that were examined. Thirty percent of the gonadotropin releasing hormone (GnRH) cells that wereexamined had ORX immunoreactive terminals in close contact with no regional or sex differences. FluoroGold injections into the preoptic area retrogradely labelled a subpopulation of ORX cells in the lateral hypothalamic/perifornical area, showing ORX cells of this region project to the preoptic andcould potentially provide input to GnRH cells. These findings suggest an integral role for ORX in the regulation of GnRH cells in the sheep and thus provide evidence of a novel mechanism whereby leptin can influence reproductive neuroendocrine function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:22:29