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Titolo:
Tissue shrinkage and unbiased stereological estimation of particle number and size
Autore:
Dorph-Petersen, KA; Nyengaard, JR; Gundersen, HJG;
Indirizzi:
Univ Aarhus, Inst Expt Clin Res, Univ Inst Pathol, Stereol Res Lab, DK-8000 Aarhus C, Denmark Univ Aarhus Aarhus Denmark C Stereol Res Lab, DK-8000 Aarhus C, Denmark Univ Aarhus, Inst Expt Clin Res, Univ Inst Pathol, Electron Microscopy Lab, DK-8000 Aarhus, Denmark Univ Aarhus Aarhus Denmark DK-8000 croscopy Lab, DK-8000 Aarhus, Denmark
Titolo Testata:
JOURNAL OF MICROSCOPY-OXFORD
, volume: 204, anno: 2001,
parte:, 3
pagine: 232 - 246
SICI:
0022-2720(200112)204:<232:TSAUSE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOL METHACRYLATE; ARBITRARY PARTICLES; HUMAN-BRAIN; VERTICAL SECTIONS; FINAL DIMENSIONS; REFRACTIVE-INDEX; SURFACE-AREA; NEURONS; DISECTOR; RAT;
Keywords:
deformation; disector; fractionator; number estimation; section thickness; shrinkage; size estimation; stereology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Dorph-Petersen, KA Univ Pittsburgh, Dept Psychiat, W1651 BST,3811 Ohara St, Pittsburgh, PA 15213 USA Univ Pittsburgh W1651 BST,3811 Ohara St Pittsburgh PA USA 15213
Citazione:
K.A. Dorph-Petersen et al., "Tissue shrinkage and unbiased stereological estimation of particle number and size", J MICROSC O, 204, 2001, pp. 232-246

Abstract

This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paperintroduces new estimators to be used in optical fractionator and optical disector designs: the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:27:52