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Titolo:
Human IgA activates the complement system via the mannan-binding lectin pathway
Autore:
Roos, A; Bouwman, LH; van Gijlswijk-Janssen, DJ; Faber-Krol, MC; Stahl, GL; Daha, MR;
Indirizzi:
Leiden Univ, Med Ctr, Dept Nephrol, NL-2300 RC Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RC NL-2300 RC Leiden, Netherlands Harvard Univ, Sch Med,Ctr Expt Therapeut & Reperfus Injury, Brigham & Womens Hosp, Dept Anesthesiol Perioperat & Pain Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ioperat & Pain Med, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 5, volume: 167, anno: 2001,
pagine: 2861 - 2868
SICI:
0022-1767(20010901)167:5<2861:HIATCS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SERUM IGA; POLYMERIC IGA; ALTERNATIVE PATHWAY; SERINE-PROTEASE; MONOMERIC IGA; GLOMERULAR DEPOSITION; RHEUMATOID-ARTHRITIS; IMMUNOGLOBULIN-A; SECRETORY IGA; MANNOSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Roos, A Leiden Univ, Med Ctr, Dept Nephrol, D3P,Postbox 9600, NL-2300 RC Leiden, Netherlands Leiden Univ D3P,Postbox 9600 Leiden Netherlands NL-2300RC rlands
Citazione:
A. Roos et al., "Human IgA activates the complement system via the mannan-binding lectin pathway", J IMMUNOL, 167(5), 2001, pp. 2861-2868

Abstract

The recently identified lectin pathway of the complement system, initiatedby binding of mannan-binding lectin (MBL) to its ligands, is a key component of innate immunity. MBL-deficient individuals show an increased susceptibility for infections, especially of the mucosal system. We examined whether IgA, an important mediator of mucosal immunity, activates the complement system via the lectin pathway. Our results indicate a dose-dependent binding of MBL to polymeric, but not monomeric IgA coated in microliter plates. This interaction involves the carbohydrate recognition domain of MBL, because it was calcium dependent and inhibited by mannose and by mAb against thisdomain of MBL. Binding of MBL to IgA induces complement activation, as demonstrated by a dose-dependent deposition of C4 and C3 upon addition of a complement source. The MBL concentrations required for IgA-induced C4 and C3 activation are well below the normal MBL plasma concentrations. In line with these experiments, serum from individuals having mutations in the MBL gene showed significantly less activation of C4 by IgA and mannan than serum from wild-type individuals. We conclude that MBL binding to IgA results in complement activation, which is proposed to lead to a synergistic action of MBL and IgA in antimicrobial defense. Furthermore, our results may explain glomerular complement deposition in IgA nephropathy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:55:53