Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A novel function of phosphorothioate oligodeoxynucleotides as chemoattractants for primary macrophages
Autore:
Baek, KH; Ha, SJ; Sung, YC;
Indirizzi:
Pohang Univ Sci & Technol, Div Mol & Life Sci, Natl Res Lab DNA Med, Pohang 790784, South Korea Pohang Univ Sci & Technol Pohang South Korea 790784 790784, South Korea
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 5, volume: 167, anno: 2001,
pagine: 2847 - 2854
SICI:
0022-1767(20010901)167:5<2847:ANFOPO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASES; ANTIGEN-PRESENTING CELLS; CPG OLIGODEOXYNUCLEOTIDES; BACTERIAL-DNA; IMMUNOSTIMULATORY DNA; SULFUR-DIOXIDE; CUTTING EDGE; B-CELL; IL-12 PRODUCTION; DENDRITIC CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Sung, YC Pohang Univ Sci & Technol, Div Mol & Life Sci, Natl Res Lab DNA Med, Pohang 790784, South Korea Pohang Univ Sci & Technol Pohang South Korea 790784 South Korea
Citazione:
K.H. Baek et al., "A novel function of phosphorothioate oligodeoxynucleotides as chemoattractants for primary macrophages", J IMMUNOL, 167(5), 2001, pp. 2847-2854

Abstract

Phosphorothioate cytosine-guanine oligodeoxynucleotides (CpG PS-ODNs) has been reported to induce Th1 immune responses against coadministered Ags more efficiently than phosphodiester CpG ODNs (CpG PO-ODNs). Here, we demonstrated that PS-ODNs, but not PO-ODNs, have a chemotactic effect on primary macrophages, which is independent of the CpG motif. In addition, the conjugation of a hexameric dG run (dG(6) run) at the 3' terminus reduced the concentration required for the optimal chemotactic activity of PS-ODNs by similarto 10-fold. Endosomal maturation blockers, such as monensin and chloroquine, inhibited the chemotactic effect of PS-ODNs. The inhibition of the activities of p38 mitogen-activated protein (MAP) kinase, and extracellular signal-related kinases (ERKs) as well as phosphoinositide 3-kinase with their specific inhibitors also resulted in suppressing the chemotaxis of primary macrophages induced by PS-ODNs. These results indicate that the PS-ODN-mediated chemotaxis requires the activation of ERKs, p38 MAP kinase, and phosphoinositide 3-kinase as well as endosomal maturation. In addition, the phosphorylations of the p38 MAP kinase, ERKs, and protein kinase B, Akt, were induced by PS-ODN, which were further enhanced by the presence of both a dG(6)run and CpG motifs. Our findings suggest that the chemotactic activity of PS-ODNs may be one of the mechanisms by which PS-ODNs exhibit stronger immunomodulatory activities than PO-ODNs in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 02:42:14