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Titolo:
Vpr is preferentially targeted by CTL during HIV-1 infection
Autore:
Altfeld, M; Addo, MM; Eldridge, RL; Yu, XG; Thomas, S; Khatri, A; Strick, D; Phillips, MN; Cohen, GB; Islam, SA; Kalams, SA; Brander, C; Goulder, PJR; Rosenberg, ES; Walker, BD;
Indirizzi:
Massachusetts Gen Hosp, Partners AIDS Res Ctr, Boston, MA USA Massachusetts Gen Hosp Boston MA USA rtners AIDS Res Ctr, Boston, MA USA Massachusetts Gen Hosp, Div Infect Dis, Boston, MA USA Massachusetts Gen Hosp Boston MA USA osp, Div Infect Dis, Boston, MA USA Massachusetts Gen Hosp, Endocrine Unit, Boston, MA USA Massachusetts Gen Hosp Boston MA USA osp, Endocrine Unit, Boston, MA USA Harvard Univ, Sch Med, Boston, MA 02129 USA Harvard Univ Boston MA USA 02129 vard Univ, Sch Med, Boston, MA 02129 USA China Med Univ, Affiliated Hosp 1, Ctr AIDS Res, Shenyang, Peoples R ChinaChina Med Univ Shenyang Peoples R China Res, Shenyang, Peoples R China John Radcliffe Hosp, Nuffield Dept Med, Oxford OX3 9DU, England John Radcliffe Hosp Oxford England OX3 9DU Med, Oxford OX3 9DU, England Brigham & Womens Hosp, Dept Med, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 Dept Med, Boston, MA 02115 USA Harvard Univ, Sch Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 vard Univ, Sch Med, Boston, MA 02115 USA Fenway Community Hlth Ctr, Boston, MA 02116 USA Fenway Community Hlth CtrBoston MA USA 02116 h Ctr, Boston, MA 02116 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 5, volume: 167, anno: 2001,
pagine: 2743 - 2752
SICI:
0022-1767(20010901)167:5<2743:VIPTBC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; T-LYMPHOCYTE RESPONSES; CELL-CYCLE ARREST; NUCLEAR-LOCALIZATION; MUTATIONAL ANALYSIS; VIF PROTEIN; VIRION INCORPORATION; FINE SPECIFICITY; PROVIRAL DNA; G(2) ARREST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Walker, BD Massachusetts Gen Hosp E, CNY 5212,149 13th St, Charlestown, MA02129 USA Massachusetts Gen Hosp E CNY 5212,149 13th St Charlestown MA USA02129
Citazione:
M. Altfeld et al., "Vpr is preferentially targeted by CTL during HIV-1 infection", J IMMUNOL, 167(5), 2001, pp. 2743-2752

Abstract

The HIV-1 accessory proteins Vpr, Vpu, and Vif are essential for viral replication, and their cytoplasmic production suggests that they should be processed for recognition by CTLs. However, the extent to which these proteinsare targeted in natural infection, as well as precise CTL epitopes within them, remains to be defined. In this study, CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in 60 HIV-1-infected individuals and 10 HIV-1-negative controls using overlapping peptides spanning the entire proteins. Peptide-specific IFN-gamma production was measured by ELISPOT assay and flow-based intracellular cytokine quantification. HLA class I restriction and cytotoxic activity were confirmed after isolation of peptide-specific CD8(+) T cell lines. CD8(+) T cell responses against Vpr, Vpu, and Vif were found in 45%, 2%, and 33% of HIV-1-infected individuals, respectively. MultipleCTL epitopes were identified in functionally important regions of HIV-1 Vpr and Vif. Moreover, in infected individuals in whom the breadth of HIV-1-specific responses was assessed comprehensively, Vpr and p17 were the most preferentially targeted proteins per unit length by CD8(+) T cells. These data indicate that despite the small size of these proteins Vif and Vpr are frequently targeted by CTL in natural HIV-1 infection and contribute importantly to the total HIV-1-specific CD8(+) T cell responses. These findings will be important in evaluating the specificity and breadth of immune responses during acute and chronic infection, and in the design and testing of candidate HIV vaccines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:18:26