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Titolo:
Role of CD47 in the induction of human naive T cell anergy
Autore:
Avice, MN; Rubio, M; Sergerie, M; Delespesse, G; Sarfati, M;
Indirizzi:
Univ Montreal, Notre Dame Hosp, Ctr Rech Ctr Hosp, Allergy Lab Res, Montreal, PQ H2L 4M1, Canada Univ Montreal Montreal PQ Canada H2L 4M1 es, Montreal, PQ H2L 4M1, Canada Univ Montreal, Dept Obstet & Gynecol, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 5, volume: 167, anno: 2001,
pagine: 2459 - 2468
SICI:
0022-1767(20010901)167:5<2459:ROCITI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTEGRIN-ASSOCIATED PROTEIN; CLONAL ANERGY; IN-VIVO; IL-2 RECEPTOR; TOLERANCE; CTLA-4; LYMPHOCYTES; INHIBITION; PROLIFERATION; STIMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Sarfati, M Univ Montreal, Notre Dame Hosp, Ctr Rech Ctr Hosp, Allergy Lab Res, M4211K,1560 Sherbrooke St E, Montreal, PQ H2L 4M1, Canada Univ Montreal M4211K,1560 Sherbrooke St E Montreal PQ Canada H2L 4M1
Citazione:
M.N. Avice et al., "Role of CD47 in the induction of human naive T cell anergy", J IMMUNOL, 167(5), 2001, pp. 2459-2468

Abstract

We recently reported that CD47 ligation inhibited IL-2 release by umbilical cord blood mononuclear cells activated in the presence of IL-12, but not IL-4, preventing the induction of IL-12R beta (2) expression and the acquisition of Th1, but not the Th2 phenotype. Here we show that in the absence of exogenous cytokine at priming, CD47 ligation of umbilical cord blood mononuclear cells promotes the development of hyporesponsive T cells. Naive cells were treated with CD47 mAb for 3 days, expanded in IL-2 for 9-12 days, and restimulated by CD3 and CD28 coengagement. Effector T cells generated under these conditions were considered to be anergic because they produced a reduced amount of IL-2 at the single-cell level and displayed an impaired capacity 1) to proliferate, 2) to secrete Th1/Th2 cytokines, and 3) to respond to IL-2, IL-4, or IL-12. Moreover, CD47 mAb strongly suppressed IL-2 production and IL-2R alpha expression in primary cultures and IL-2 response ofactivated naive T cells. Induction of anergy by CD47 mAb was IL-10 independent, whereas inclusion of IL-2 and IL-4, but not IL-7, at priming fully restored T cell activation. Furthermore, CD28 costimulation prevented induction of anergy. Thus, CD47 may represent a potential target to induce anergy and prevent undesired Th0/Th1 responses such as graft vs host diseases, allograft rejection, or autoimmune diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:31:11