Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Monoclonal antibodies with broad specificity for hepatitis C virus hypervariable region 1 variants can recognize viral particles
Autore:
Cerino, A; Meola, A; Segagni, L; Furione, M; Marciano, S; Triyatni, M; Liang, TJ; Nicosia, A; Mondelli, MU;
Indirizzi:
IRCCS Policlin San Mateo, Lab Riv Area Infettivol, I-27100 Pavia, Italy IRCCS Policlin San Mateo Pavia Italy I-27100 tivol, I-27100 Pavia, Italy IRCCS Policlin San Mateo, Dipartimento Malattie Infett, I-27100 Pavia, Italy IRCCS Policlin San Mateo Pavia Italy I-27100 nfett, I-27100 Pavia, Italy IRCCS Policlin San Mateo, Serv Virol, I-27100 Pavia, Italy IRCCS Policlin San Mateo Pavia Italy I-27100 Virol, I-27100 Pavia, Italy Univ Pavia, I-27100 Pavia, Italy Univ Pavia Pavia Italy I-27100Univ Pavia, I-27100 Pavia, Italy Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Italy Ist Ric Biol Mol P Angeletti Pomezia Italy I-00040 -00040 Pomezia, Italy NIDDKD, Liver Dis Sect, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 Liver Dis Sect, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 167, anno: 2001,
pagine: 3878 - 3886
SICI:
0022-1767(20011001)167:7<3878:MAWBSF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL EPITOPE; IMMUNE-RESPONSE; INSECT CELLS; IN-VITRO; INFECTION; CHIMPANZEES; SEQUENCE; BINDING; CD81; E2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Mondelli, MU IRCCS Policlin San Mateo, Lab Riv Area Infettivol, Via Taramelli 5, I-27100 Pavia, Italy IRCCS Policlin San Mateo Via Taramelli 5 PaviaItaly I-27100
Citazione:
A. Cerino et al., "Monoclonal antibodies with broad specificity for hepatitis C virus hypervariable region 1 variants can recognize viral particles", J IMMUNOL, 167(7), 2001, pp. 3878-3886

Abstract

The hypervariable region 1 (HVR1) of the E2 protein of hepatitis C virus (HCV) is a highly heterogeneous sequence that is promiscuously recognized byhuman sera via binding to amino acid residues with conserved physicochemical properties. We generated a panel of mAbs from mice immunized with HVR1 surrogate peptides (mimotopes) affinity-selected with sera from HCV-infectedpatients from a phage display library. A high number of specific clones was obtained after immunization with a pool of nine mimotopes, and the resulting mAbs were shown to recognize several 16- and 27-mer peptides derived from natural HVR1 sequences isolated from patients with acute and chronic HCVinfection, suggesting that HVR1 mimotopes were efficient antigenic and immunogenic mimics of naturally occurring HCV variants. Moreover, most mAbs were shown to bind HVR1 in the context of a complete soluble form of the E2 glycoprotein, indicating recognition of correctly folded HVR1. In addition, a highly promiscuous mAb was able to specifically capture bona fide viral particles (circulating HCV RNA) as well as rHCV-like particles assembled in insect cells expressing structural viral polypeptides derived from an HCV la isolate. These findings demonstrate that it is possible to induce a broadly cross-reactive clonal Ab response to multiple HCV variants. In consideration of the potentially important role of HVR1 in virus binding to cellularreceptor(s), such a mechanism could be exploited for induction of neutralizing Abs specific for a large repertoire of viral variants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:20:54