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Titolo:
Both CD34(+)38(+) and CD34(+)38(-) cells home specifically to the bone marrow of NOD/LtSZ scid/scid mice but show different kinetics in expansion
Autore:
Kerre, TCC; De Smet, G; De Smedt, M; Offner, F; De Bosscher, J; Plum, J; Vandekerckhove, B;
Indirizzi:
State Univ Ghent Hosp, Dept Clin Chem Microbiol & Immunol, B-9000 Ghent, Belgium State Univ Ghent Hosp Ghent Belgium B-9000 mmunol, B-9000 Ghent, Belgium State Univ Ghent Hosp, Dept Hematol, B-9000 Ghent, Belgium State Univ Ghent Hosp Ghent Belgium B-9000 ematol, B-9000 Ghent, Belgium Bloedtransfusiecentrum Oost Vlaanderen, Ghent, Belgium Bloedtransfusiecentrum Oost Vlaanderen Ghent Belgium en, Ghent, Belgium
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 167, anno: 2001,
pagine: 3692 - 3698
SICI:
0022-1767(20011001)167:7<3692:BCACCH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; UMBILICAL-CORD BLOOD; MOBILIZED PERIPHERAL-BLOOD; RECEPTOR-BETA-CHAIN; IMMUNE-DEFICIENT MICE; EX-VIVO EXPANSION; PROGENITOR CELLS; SCID MICE; IMMUNODEFICIENT MICE; STEM-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Kerre, TCC State Univ Ghent Hosp, Dept Clin Chem Microbiol & Immunol, 4BlokA,De Pintelaan 185, B-9000 Ghent, Belgium State Univ Ghent Hosp 4BlokA,De Pintelaan 185 Ghent Belgium B-9000
Citazione:
T.C.C. Kerre et al., "Both CD34(+)38(+) and CD34(+)38(-) cells home specifically to the bone marrow of NOD/LtSZ scid/scid mice but show different kinetics in expansion", J IMMUNOL, 167(7), 2001, pp. 3692-3698

Abstract

Human hemopoietic stem cells (HSC) have been shown to engraft, differentiate, and proliferate in the hemopoietic tissues of sublethally irradiated NOD/LtSZ scid/scid (NOD/SCID) mice. We used this model to study homing, survival, and expansion of human HSC populations from different sources or phenotype. We observed that CD34(+) cells homed specifically to bone marrow (BM)and spleen, but by 3 days after injection, survived only in the BM. These BM-homed CD34(+) cells proliferated intensively and gave rise to a 12-fold,5.5-fold, and 4-fold expansion in 3 days for umbilical cord blood, adult mobilized peripheral blood, and adult BM-derived cells, respectively. By injection of purified subpopulations, it was demonstrated that both CD34(+)38() and CD34(+)38(-) umbilical cord blood HSC homed to the BM and expanded. Importantly, kinetics of expansion were different: CD34(+)38(+) cells started to increase in cell number from day 3 onwards, and by 4 wk after injection, virtually all CD34(+) cells had disappeared. In contrast, CD34(+)38(-) cells remained quiescent during the first week and started to expand intensively from the third week on. In this paper, we have shown that homing, survival, and expansion of stem cells are three independent phenomena important in the early phase of BM engraftment and that kinetics of engraftment differ between CD34(+)38(+) and CD34(+)38(-) cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:12:58