Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IL-10 inhibits granulocyte-macrophage colony-stimulating factor-dependent human monocyte survival at the early stage of the culture and inhibits the generation of macrophages
Autore:
Hashimoto, S; Komuro, I; Yamada, M; Akagawa, KS;
Indirizzi:
Natl Inst Infect Dis, Dept Immunol, Shinjuku Ku, Tokyo 1628640, Japan NatlInst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Univ Tokyo, Sch Med, Dept Mol Prevent Med, Tokyo 113, Japan Univ Tokyo Tokyo Japan 113 h Med, Dept Mol Prevent Med, Tokyo 113, Japan Morinaga Milk Ind Co Ltd, Biochem Res Lab, Kanagawa, Japan Morinaga Milk Ind Co Ltd Kanagawa Japan iochem Res Lab, Kanagawa, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 7, volume: 167, anno: 2001,
pagine: 3619 - 3625
SICI:
0022-1767(20011001)167:7<3619:IIGCFH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTINUCLEATED GIANT-CELLS; HUMAN T-CELLS; CELLULAR CYTOTOXICITY; CYTOKINE SYNTHESIS; DNA FRAGMENTATION; DENDRITIC CELLS; TNF-ALPHA; B-CELLS; GM-CSF; INTERLEUKIN-10;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Akagawa, KS Natl Inst Infect Dis, Dept Immunol, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628640, Japan Natl Inst Infect Dis 1-23-1 Toyama Tokyo Japan 1628640 Japan
Citazione:
S. Hashimoto et al., "IL-10 inhibits granulocyte-macrophage colony-stimulating factor-dependent human monocyte survival at the early stage of the culture and inhibits the generation of macrophages", J IMMUNOL, 167(7), 2001, pp. 3619-3625

Abstract

We previously demonstrated that IL-10 alone does not stimulate growth and differentiation of human monocytes, but enhances those of monocytes stimulated with M-CSF. We studied here the effect of IL-10 on human monocytes stimulated with GM-CSF. Monocytes stimulated with GM-CSF alone survived and developed into macrophages. Monocytes cultured with GM-CSF plus IL-10, however, died through apoptosis. IL-10 decreased expression of bcl-2, bcl-x(L), and mcl-1- but not box mRNA in monocytes stimulated with GM-CSF. IL-10 did not change the expression of mRNA of both GM-CSFR alpha -chain and beta -chain, but inhibited tyrosine phosphorylation of STAT5 and extracellular signal-regulated kinases 1 and 2 in the monocytes. The inhibitory effect of IL-10was restricted to treatment 48 h after stimulation with GM-CSF. Addition of IL-10 after that time induced neither apoptosis nor a decrease in expression of bcl-2, bcl-x(L), and mcl-1 mRNA. IL-10, however, inhibited LPS-induced TNF-alpha production even in these cells, indicating that the cells still possessed responsiveness to IL-10. Monocytes pretreated for >48 h with GM-CSF became resistant to GM-CSF withdrawal, and the cells could survive without GM-CSF. These results indicate that IL-10 selectively inhibits GM-CSF-dependent monocyte survival by inhibiting the signaling events induced by GM-CSF, but the timing of addition of IL-10 is critical, and IL-10 had to beadded within 48 h after stimulation with GM-CSF to achieve the inhibitory effect. These results taken together with our previous results indicate that IL-10 plays a pivotal role in monocyte survival and development into macrophages in concert with M-CSF and GM-CSF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:57:29