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Titolo:
alpha(1)-adrenergic stimulation mediates Ca2+-dependent inositol phosphateformation through the alpha(1B)-like adrenoceptor subtype in adult rat cardiac myocytes
Autore:
Seraskeris, S; Lazou, A;
Indirizzi:
Aristotelian Univ Thessaloniki, Sch Biol, Dept Zool, Physiol Anim Lab, GR-54006 Thessaloniki, Greece Aristotelian Univ Thessaloniki Thessaloniki Greece GR-54006 niki, Greece
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 84, anno: 2002,
pagine: 201 - 210
SICI:
0730-2312(2002)84:1<201:ASMCIP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-1-ADRENERGIC RECEPTOR SUBTYPES; ACTIVATED PROTEIN-KINASE; VASCULAR SMOOTH-MUSCLE; ALPHA(1D)-ADRENERGIC RECEPTORS; INOTROPIC RESPONSES; MESSENGER-RNAS; CONTRACTION; MYOCARDIUM; MECHANISMS; ALPHA(1C);
Keywords:
alpha(1)-adrenoceptors; inositol phosphates; cardiac myocytes; Ca2+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Lazou, A Aristotelian Univ Thessaloniki, Sch Biol, Dept Zool, Physiol AnimLab, GR-54006 Thessaloniki, Greece Aristotelian Univ Thessaloniki Thessaloniki Greece GR-54006 ece
Citazione:
S. Seraskeris e A. Lazou, "alpha(1)-adrenergic stimulation mediates Ca2+-dependent inositol phosphateformation through the alpha(1B)-like adrenoceptor subtype in adult rat cardiac myocytes", J CELL BIOC, 84(1), 2002, pp. 201-210

Abstract

We studied the effects of increased Ca2+ influx on alpha (1)-adrenoceptor-stimulated InsP formation in adult rat cardiac myocytes. We further examined if such effects could be mediated through a specific alpha (1)-adrenoceptor subtype. [H-3]InsP responses to adrenaline were dependent on extracellular Ca2+ concentration, from 0.1 muM to 2 mM, and were completely blocked byCa2+ removal. However, in cardiac myocytes preloaded with BAPTA, a highly selective calcium chelating agent, Ca2+ concentrations higher than 1 muM had no effect on adrenaline-stimulated [H-3]InsP formation. Taken together these results suggest that [H-3]InsP formation induced by alpha (1)-adrenergic stimulation is in part mediated by increased Ca2+ influx. Consistent withthis, ionomycin, a calcium ionophore, stimulated [H-3]InsP formation. Thisresponse was additive with the response to adrenaline stimulation implyingthat different signaling mechanisms may be involved. In cardiac myocytes treated with the alpha (1B)-adrenoceptor alkylating agent, CEC, [H-3]InsP formation remained unaffected by increased Ca2+ concentrations, a pattern similar to that observed when intracellular Ca2+ was chelated with BAPTA. In contrast, addition of the alpha (1A)-subtype antagonist, 5'-methyl urapidil,did not affect the Ca2+ dependence of [H-3]InsP formation. Neither nifedipine, a voltage-dependent Ca2+ channel blocker nor the inorganic Ca2+ channel blockers, Ni2+ and Co2+, had any effect on adrenaline stimulated [H-3]InsP, at concentrations that inhibit Ca2+ channels. The results suggest that in adult rat cardiac myocytes, in addition to G protein-mediated response, alpha (1)-adrenergic-stimulated [3H]InsP formation is activated by increasedCa 2 - influx mediated by the alpha-(1B)-subtype. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:28:38