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Titolo:
Suppression of cell proliferation and deoxyribonucleic acid synthesis in cloned rat hepatoma H4-II-E cells overexpressing regucalcin
Autore:
Misawa, H; Inagaki, S; Yamaguchi, M;
Indirizzi:
Univ Shizouka, Grad Sch Nutr Sci, Lab Endocrinol & Mol Metab, Shizuoka 4228526, Japan Univ Shizouka Shizuoka Japan 4228526 Mol Metab, Shizuoka 4228526, Japan
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 84, anno: 2002,
pagine: 143 - 149
SICI:
0730-2312(2002)84:1<143:SOCPAD>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN REGUCALCIN; ENDOGENOUS REGUCALCIN; TRANSCRIPTIONAL REGULATION; PHOSPHATASE REGULATION; SIGNALING FACTORS; MESSENGER-RNA; LIVER; NUCLEI; INVOLVEMENT; EXPRESSION;
Keywords:
regucalcin; cell proliferation; DNA synthesis; cloned rat hepatoma H4-II-E cells; transfectant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, M Univ Shizouka, Grad Sch Nutr Sci, Lab Endocrinol & Mol Metab,52-1 Yada, Shizuoka 4228526, Japan Univ Shizouka 52-1 Yada Shizuoka Japan4228526 228526, Japan
Citazione:
H. Misawa et al., "Suppression of cell proliferation and deoxyribonucleic acid synthesis in cloned rat hepatoma H4-II-E cells overexpressing regucalcin", J CELL BIOC, 84(1), 2002, pp. 143-149

Abstract

The role of endogenous regucalcin (RC) in the regulation of cell proliferation was investigated in the cloned rat hepatoma H4-II-E cells overexpressing RC stably. H4-II-E cells were transfected with RC/pCXN2 vector and the multiple neomycin-resistant clones which overexpress stably RC were selected. The RC content of RC/pCXN2-transfected cells used in this study was 19.7-fold as compared with that of the parental wild type H4-II-E cells. Wi Id type H4-II-E cells, pCXN2 vector-transfected cel Is (mock type), and RC/pCXN2-transfected cells (transfectants) were cultured for 24, 48, and 72 h in the presence of fetal bovine serum (10% FBS). Cell numbers of wild and mock type were significantly increased with the time course of culture. Cell numbers of transfectants was significantly suppressed as compared with that ofwild and mock type. Deoxyribonucleic acid (DNA) synthesis activity in the nuclear fraction of H4-II-E cells was significantly suppressed in transfectants with culture for 12-48 h. The presence of anti-RC monoclonal antibody (10-50 ng/ml) in the reaction mixture caused a significant increase in DNA synthesis activity in the nuclei of wild type and transfectants; this increase was remarkable in transfectants. The effect of anti-RC monoclonal antibody (50 ng/ml) in increasing DNA synthesis activity in transfectants was completely prevented by the addition of regucalcin (1 muM). This study demonstrates that cell proliferation is suppressed in the cloned rat hepatoma H4-II-E overexpressing RC stably. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:03:15