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Titolo:
Changes in the expression of annexin A5 gene during in vitro chondrocyte differentiation: Influence of cell attachment
Autore:
Turnay, J; Olmo, N; Lizarbe, MA; von der Mark, K;
Indirizzi:
Univ Complutense, Fac Chem, Dept Biochem & Mol Biol 1, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 Mol Biol 1, E-28040 Madrid, Spain Univ Erlangen Nurnberg, Nikolaus Fiebiger Ctr Mol Med, Dept Expt Med 1, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 84, anno: 2002,
pagine: 132 - 142
SICI:
0730-2312(2002)84:1<132:CITEOA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN-BINDING-PROTEIN; GROWTH-PLATE CARTILAGE; VESICLE-MEDIATED MINERALIZATION; ANCHORIN-CII; X COLLAGEN; MATRIX VESICLES; CHICK-EMBRYO; OSTEOARTHRITIC CARTILAGE; II COLLAGEN; V GENE;
Keywords:
hypertrophic chondrocyte; chicken sterna; extracellular matrix; collagen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Lizarbe, MA Univ Complutense, Fac Chem, Dept Biochem & Mol Biol 1, E-28040Madrid, Spain Univ Complutense Madrid Spain E-28040 E-28040 Madrid, Spain
Citazione:
J. Turnay et al., "Changes in the expression of annexin A5 gene during in vitro chondrocyte differentiation: Influence of cell attachment", J CELL BIOC, 84(1), 2002, pp. 132-142

Abstract

Several lines of evidence indicate that annexin A5, a membrane-associated protein with calcium-channel activity, plays a key role in cartilage calcification during endochondral ossification. As a major constituent of cartilage matrix vesicles, which are released from microvilli of hypertrophic chondrocytes, it is involved in calcium uptake necessary for the initial stagesof cartilage calcification. Little is known, however, concerning transcriptional regulation of the annexin A5 gene during chondrocyte differentiation. Here, we report on changes in annexin A5 expression by measuring mRNA andprotein levels during in vitro differentiation of chick sternal chondrocytes to tile hypertrophic phenotype. Terminal differentiation of mature sternal chondrocytes was achieved in the presence of sodium ascorbate in high-density cultures growing either in monolayer or over agarose as cell aggregates. Differentiation of chondrocytes to hypertrophic cells was followed by morphological analysis and by the onset of type X collagen expression. High expression levels of annexin A5 mRNA were detected in chondrocytes freshly isolated from the sterna by enzymatic digestion and subsequently in cells growing in monolayer, but annexin A5 gene transcription was rapidly downregulated when cells were grown in suspension as aggregates over agarose. However, protein levels did not decrease probably due to its low turnover rate. In suspension culture, annexin A5 mRNA reappeared after 3 weeks concomitantly with segregation of the aggregates into single cells and onset of chondrocyte hypertrophy. Tile downregulation of annexin A5 expression in cells growing as matrix-rich aggregates was reverted when extracellular matrix components were removed and cells were reseeded onto tissue culture plastic, suggesting that cull spreading, formation of focal contacts and stress fibersstimulated annexin A5 expression in proliferating as well as in hypertrophic chondrocytes. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 16:49:36