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Titolo:
Phosphoinositide-specific phospholipase C beta 1 expression is not linked to nerve growth factor-induced differentiation, cell survival or cell cyclecontrol in PC12 rat pheocromocytoma cells
Autore:
Bortul, R; Aluigi, M; Tazzari, PL; Tabellini, G; Baldini, G; Bareggi, R; Narducci, P; Martelli, AM;
Indirizzi:
Univ Trieste, Dipartimento Morfol Umana Normale, I-34138 Trieste, Italy Univ Trieste Trieste Italy I-34138 Umana Normale, I-34138 Trieste, Italy Univ Bologna, Dipartimento Sci Anat Umane & Fisiopatol Apparato, Sez Anat,I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 rato, Sez Anat,I-40126 Bologna, Italy Policlin S Orsola, Serv Immunoematol & Trasfus, I-40138 Bologna, Italy Policlin S Orsola Bologna Italy I-40138 Trasfus, I-40138 Bologna, Italy Univ Bologna, Sch Pharm, Bologna, Italy Univ Bologna Bologna ItalyUniv Bologna, Sch Pharm, Bologna, Italy IOR, CNR, Inst Citomorfol Normale & Patol, I-40137 Bologna, Italy IOR Bologna Italy I-40137 morfol Normale & Patol, I-40137 Bologna, Italy
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 84, anno: 2002,
pagine: 56 - 67
SICI:
0730-2312(2002)84:1<56:PPCB1E>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; HUMAN NT2-N NEURONS; SOMATOSENSORY CORTEX; NEURITE OUTGROWTH; DENDRITIC GROWTH; C ISOZYMES; 3T3 CELLS; 2 FORMS; NUCLEAR; 3-KINASE;
Keywords:
cell proliferation; apoptosis; phosphoinositide hydrolysis; nucleus; localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Martelli, AM Univ Trieste, Dipartimento Morfol Umana Normale, Via Manzoni 16, I-34138 Trieste, Italy Univ Trieste Via Manzoni 16 Trieste Italy I-34138 ste, Italy
Citazione:
R. Bortul et al., "Phosphoinositide-specific phospholipase C beta 1 expression is not linked to nerve growth factor-induced differentiation, cell survival or cell cyclecontrol in PC12 rat pheocromocytoma cells", J CELL BIOC, 84(1), 2002, pp. 56-67

Abstract

Recent reports have highlighted that phosphoinositicle-specific phospholipase C beta1 expression is linked to neuronal differentiation in different experimental models. We sought to determine whether or not this is also truefor nerve growth factor (NGF)-induced neuronal differentiation of rat PC12cells. However, we did not find differences in the expression of both the forms of phosphoinositicle-specific phospholipase C beta1 (a and b) during sympathetic differentiation of these cells. Also, PC12 cell clones stably overexpressing phosphoinositicle-specific phospholipase CPI were not more susceptible to the differentiating effect of NCF. Furthermore, since it is well established that phosphoinositide-specific phospholipase C beta1 affectscell proliferation, we investigated whether or not PC12 cell clones stablyoverexpressing phosphoinositicle-specific phospholipase C beta1 showed differences in survival to serum deprivation and cell cycle, when compared to wild type cells. Nevertheless, we did not find any differences in these parameters between wild type cells and the overexpressing clones. Interestingly, in PC12 cells the overexpressed phosphoinositide-specific phospholipase C beta1 did not localize to the nucleus, but by immunofluorescence analysis, was detected in the cytoplasm. Therefore, our findings may represent another important clue to the fact that only when it is located within the nucleus phosphoinositide-specific phospholipase C beta1 is able to influence cell proliferation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:56:37