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Titolo:
Polypeptide growth factors and phorbol ester induce progressive ankylosis (Ank) gene expression in murine and human fibroblasts
Autore:
Guo, Y; Hsu, DKW; Feng, SLY; Richards, CM; Winkles, JA;
Indirizzi:
Amer Red Cross, Holland Lab, Dept Vasc Biol, Rockville, MD 20855 USA Amer Red Cross Rockville MD USA 20855 Vasc Biol, Rockville, MD 20855 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 84, anno: 2002,
pagine: 27 - 38
SICI:
0730-2312(2002)84:1<27:PGFAPE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE PROGRESSION; RAS TRANSFORMATION; ANIMAL-MODEL; ENCODES; PROTEIN; KINASE; MICE; IDENTIFICATION; ASSOCIATION; STIMULATION;
Keywords:
fibroblast growth factor; phorbol ester; ankylosis; craniometaphyseal dysplasia; inorganic pyrophosphate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Winkles, JA Amer Red Cross, Holland Lab, Dept Vasc Biol, 15601 Crabbs Branch Way, Rockville, MD 20855 USA Amer Red Cross 15601 Crabbs Branch Way Rockville MD USA 20855
Citazione:
Y. Guo et al., "Polypeptide growth factors and phorbol ester induce progressive ankylosis (Ank) gene expression in murine and human fibroblasts", J CELL BIOC, 84(1), 2002, pp. 27-38

Abstract

Polypeptide growth factors promote cellular proliferation by binding to specific plasma membrane-anchored receptors. This interaction triggers the phosphorylation of signal transducing molecules and the transcriptional activation of numerous genes. We have used a differential display approach to identify fibroblast growth factor (FGF)-1-inducible genes in murine NIH 3T3 fibroblasts. Here we report that one of these genes encodes ank, a type IIIatransmembrane protein reported to function in cells as an inorganic pyrophosphate transporter. FGF-1 induction of ank mRNA expression is first detectable at 2 h after growth factor addition and is dependent on de novo RNA and protein synthesis. Ank gene expression is also upregulated after treatingquiescent fibroblasts with several other mitogenic agents (e.g., calf serum or platelet-derived growth factor-BB) or the tumor promoter phorbol 12-myristate 13-acetate. Furthermore, in comparison to parental NIH 3T3 cells, oncogene-transformed NIH 3T3 cells constitutively express elevated levels ofank mRNA. FGF-1 also increases ank gene expression in non-immortalized human embryonic lung fibroblasts. Finally, the murine and human ank genes are expressed in vivo in a tissue-specific manner, with highest levels of mRNA expression found in brain, heart, and skeletal muscle. These results indicate that ank is a growth factor-regulated delayed-early response gene in mammalian cells, and we propose that increased ank expression during cell cycle progression may be necessary to maintain proper intracellular pyrophosphate levels during conditions of high cellular metabolic activity. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/12/19 alle ore 15:06:55