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Titolo:
In vitro stability of a novel compliant poly(carbonate-urea) urethane to oxidative and hydrolytic stress
Autore:
Salacinski, HJ; Tai, NR; Carson, RJ; Edwards, A; Hamilton, G; Seifalian, AM;
Indirizzi:
Univ Coll London, Royal Free & Univ Coll Med Sch, Dept Surg, Tissue Engn Labs, London NW3 2QG, England Univ Coll London London England NW3 2QG gn Labs, London NW3 2QG, England Cardiotech Int Ltd, Wrexham, Wales Cardiotech Int Ltd Wrexham WalesCardiotech Int Ltd, Wrexham, Wales
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 2, volume: 59, anno: 2002,
pagine: 207 - 218
SICI:
0021-9304(200202)59:2<207:IVSOAN>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR GRAFT; IN-VITRO; POLYURETHANE ELASTOMERS; CHOLESTEROL ESTERASE; LYSOSOMAL-ENZYMES; NEW-GENERATION; DEGRADATION; INVITRO; BIODEGRADATION; BIOSTABILITY;
Keywords:
vascular grafts; compliant poly(carbonateurea)urethane; viscoelasticity; hydrolytic, oxidative, peroxidative and plasma-fraction-led degradation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Seifalian, AM Univ Coll London, Royal Free & Univ Coll Med Sch, Dept Surg,Tissue Engn Labs, Pond St, London NW3 2QG, England Univ Coll London Pond St London England NW3 2QG QG, England
Citazione:
H.J. Salacinski et al., "In vitro stability of a novel compliant poly(carbonate-urea) urethane to oxidative and hydrolytic stress", J BIOMED MR, 59(2), 2002, pp. 207-218

Abstract

Poly(ester) urethane and poly(ether)urethane vascular grafts fail in vivo because of hydrolytic and oxidative degradative mechanisms. Studies have shown that poly(carbonate)urethanes have enhanced resistance. There is still a need for a viable, nonrigid, small-diameter, synthetic vascular graft. Inthis study, we sought to confirm this by exposing a novel formulation of compliant poly(carbonate)urethane (CPU) manufactured by an innovative process, resulting in a stress-free. Small-diameter prosthesis, and a conventional poly(ether) urethane Pulse-Tec graft known to readily undergo oxidation in a variety of degradative solutions, and we assessed them for the development of oxidative and hydrolytic degradation, changes in elastic properties,and chemical stability. To simulate the in vivo environment, we used buffered solutions of phospholipase A(2) and cholesterol esterase; solutions of H2O2/CoCl2, t-butyl peroxide/CoCl2 (t-but/CoCl2), and glutathione/t-butyl peroxide/CoCl2 (Glut/t-but/CoCl2); and plasma fractions I-IV, which were derived from fresh human plasma centrifuged in poly(ethylene glycol). To act as a negative control, both graft types were incubated in distilled water. Samples of both graft types (100 mm with a 5.0-mm inner diameter) were incubated in these solutions at 37 degreesC for 70 days before environmental scanning electron microscopy, radial tensile strength and quality control, gelpermeation chromatography, and in vitro compliance assessments were performed. Oxidative degradation was ascertained from significant changes in molecular weight with respect to a control on all Pulse-Tec grafts treated witht-but/CoCl2, Glut/ t-but/CoCl2, and plasma fractions I-III. Pulse-Tec grafts exposed to the H2O2/CoCl2 mixture had significantly greater compliance than controls incubated in distilled water (p < 0.001 at 50 mmHg). No changes in molecular weight with respect to the control were observed for the CPUsamples; only those immersed in t-but/CoCl2 and Glut/t-but,/CoCl2 showed an 11% increase in molecular weight to 108,000. Only CPU grafts treated withthe Glut/t-but/CoCl2 mixture exhibited significantly greater compliance (p< 0.05 at 50 mmHg). Overall, results from this study indicate that CPU presents a far greater chemical stability than poly(ether)urethane grafts do. 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:49:46