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Titolo:
RNA export mediated by Tap involves NXT1-dependent interactions with the nuclear pore complex
Autore:
Levesque, L; Guzik, B; Guan, TL; Coyle, J; Black, BE; Rekosh, D; Hammarskjold, ML; Paschal, BM;
Indirizzi:
Univ Virginia, Dept Biochem & Mol Genet, Ctr Cell Signaling, Hlth Sci Syst, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Myles H Thaler Ctr AIDS & Human Retrovirus Res, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Scripps Clin & Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 48, volume: 276, anno: 2001,
pagine: 44953 - 44962
SICI:
0021-9258(20011130)276:48<44953:REMBTI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONSTITUTIVE TRANSPORT ELEMENT; MESSENGER-RNA; MONOCLONAL-ANTIBODIES; BINDING-PROTEIN; REV PROTEIN; P62 COMPLEX; IDENTIFICATION; MEX67P; CTE; GLYCOPROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Paschal, BM Univ Virginia, Dept Biochem & Mol Genet, Ctr Cell Signaling, Hlth Sci Syst, Box 800577, Charlottesville, VA 22908 USA Univ Virginia Box 800577 Charlottesville VA USA 22908 2908 USA
Citazione:
L. Levesque et al., "RNA export mediated by Tap involves NXT1-dependent interactions with the nuclear pore complex", J BIOL CHEM, 276(48), 2001, pp. 44953-44962

Abstract

Nuclear export of ribonucleoprotein complexes requires cis-acting signals and recognition by receptors that mediate translocation through the nuclearpore complex. Translocation is likely to involve a series of physical interactions between the ribonucleoprotein complex and nucleoporins within the nuclear pore complex. Here, we have characterized the function of NXT1 in the context of the Tap-dependent RNA export pathway. Tap has been implicatedin the nuclear export of RNA transcripts derived from Mason-Pfizer monkey virus that contain the constitutive transport element. We demonstrate that NXT1 stimulates binding of a Tap-RNA complex to nucleoporins in vitro, and we provide mutational analysis that shows these interactions are necessary for nuclear export of an intron-containing viral mRNA in vivo. Tap containsseparate domains for binding to nucleoporins and NXT1, both of which are critical for its export function. RNA export is mediated by a heterodimer ofTap and NXT1, and the function of NXT1 on this pathway is to regulate the affinity of the Tap-RNA complex for nucleoporins within the nuclear pore complex. We propose that NXT1-dependent binding of the Tap-RNA complex to thenucleoporin p62, which we have reconstituted in vitro using recombinant proteins, represents a single step of the translocation reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:11:34