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Titolo:
The microtubule cytoskeleton participates in control of beta(2) integrin avidity
Autore:
Zhou, XM; Li, JX; Kucik, DF;
Indirizzi:
Univ Illinois, Coll Dent, Dept Oral Biol, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Dept Oral Biol, Chicago, IL 60612 USA Univ Alabama, Dept Pathol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ept Pathol, Birmingham, AL 35294 USA Birmingham Med Ctr, Vet Affairs Med Res Serv, Birmingham, AL 35294 USA Birmingham Med Ctr Birmingham AL USA 35294 Serv, Birmingham, AL 35294 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 48, volume: 276, anno: 2001,
pagine: 44762 - 44769
SICI:
0021-9258(20011130)276:48<44762:TMCPIC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTION-ASSOCIATED ANTIGEN-1; BINDING PROTEIN-RHO; C-KINASE SUBSTRATE; CELL-ADHESION; ACTIN CYTOSKELETON; TYROSINE PHOSPHORYLATION; SIGNAL-TRANSDUCTION; MONOCLONAL-ANTIBODIES; CONFORMATIONAL CHANGE; CYTOPLASMIC TAIL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Li, JX Univ Illinois, Coll Dent, Dept Oral Biol, 801 S Paulina, Chicago, IL 60612USA Univ Illinois 801 S Paulina Chicago IL USA 60612 icago, IL 60612USA
Citazione:
X.M. Zhou et al., "The microtubule cytoskeleton participates in control of beta(2) integrin avidity", J BIOL CHEM, 276(48), 2001, pp. 44762-44769

Abstract

Leukocyte avidity is regulated by cytoskeletal constraints, which keep beta (2) integrins in an inactive mode. Releasing these constraints results inincreased lateral mobility and clustering of integrins, effectively activating adhesion. At least part of the constraint on beta (2) integrins is dueto actin; whether other cytoskeletal components are involved has not previously been investigated. Microtubules are a candidate for control of integrin rearrangement, because they modulate focal adhesions, which are sites ofinteraction between integrins and the cytoskeleton. Here we report that both depolymerization of microtubules by colchicine or nocodazole and stabilization of microtubules by taxol increased the lateral mobility of beta (2) integrins, activating adhesion. Increased integrin mobility was accompaniedby an increase in tyrosine phosphorylation of paxillin, a biochemical event associated with activation of beta (2) integrins. Further, C3 exoenzyme, an inhibitor of Rho, blocked induction of integrin mobility by nocodazole, but not by taxol, suggesting that there are multiple microtubule-dependent pathways to integrin rearrangement, only some of which require Rho activity. Taken together, our data suggest that a dynamic microtubule system is required to regulate integrin-cytoskeleton interactions. Furthermore, these data demonstrate that microtubules participate in control of integrin rearrangement, one of the earliest steps in activation of integrin-mediated adhesion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 18:58:03