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Titolo:
p11 expression in human bronchial epithelial cells is increased by nitric oxide in a cGMP-dependent pathway involving protein kinase G activation
Autore:
Pawliczak, R; Cowan, MJ; Huang, XL; Nanavaty, UB; Alsaaty, S; Logun, C; Shelhamer, JH;
Indirizzi:
NIH, Dept Crit Care Med, Warren Grant Magnuson Clin Ctr, Bethesda, MD 20892 USA NIH Bethesda MD USA 20892 Grant Magnuson Clin Ctr, Bethesda, MD 20892 USA Med Univ Lodz, Dept Allergy & Clin Immunol, PL-92211 Lodz, Poland Med UnivLodz Lodz Poland PL-92211 & Clin Immunol, PL-92211 Lodz, Poland
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 48, volume: 276, anno: 2001,
pagine: 44613 - 44621
SICI:
0021-9258(20011130)276:48<44613:PEIHBE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANNEXIN-II TETRAMER; BIOCHEMICAL-CHARACTERIZATION; PHOSPHOLIPASE A(2); C-JUN; TRANSCRIPTION; BINDING; PHOSPHORYLATION; STIMULATION; SUBSTRATE; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Shelhamer, JH NIH, Dept Crit Care Med, Warren Grant Magnuson Clin Ctr, Bldg 10,Rm 7D43,9000 Rockville Pike, Bethesda, MD 20892 USA NIH Bldg 10,Rm 7D43,9000 Rockville Pike Bethesda MD USA 20892
Citazione:
R. Pawliczak et al., "p11 expression in human bronchial epithelial cells is increased by nitric oxide in a cGMP-dependent pathway involving protein kinase G activation", J BIOL CHEM, 276(48), 2001, pp. 44613-44621

Abstract

The effect of nitric oxide on p11 expression was studied in an immortalized human bronchial epithelial cell line (BEAS-2B cells). Three nitric oxide donors were used: spermine NONOate (SP), (+/-)-S-nitroso-N-acetylpenicillamine (SNAP), and S-nitrosoglutathione (SNOG). All three nitric oxide donors had similar effects resulting in dose-dependent and time-dependent accumulation of pll protein and an increase of steady-state p11 mRNA. Studies usinga reporter gene containing the region from -1499 to +89 of the pll promoter demonstrated an increase in transcriptional activity after stimulation with NO donors for 4 h. These effects were abolished at the promoter and protein level using protein kinase G inhibitors (KT5823 and R-p-8-pCPT-cGMPS). Incubation of transfected cells with a cell permeable cGMP analogue (8-Br-cGMP) resulted in a dose-related increase of promoter activity. An electrophoretic mobility shift assay of nuclear proteins extracted from BEAS-2B cells identified an AP-1 site located at -82 to -77 of the p11 promoter region as an NO- and cGMP- dependent response element. These data were confirmed using a c-jun dominant negative mutant vector and a e-jun expression plasmid. Therefore, we conclude that nitric oxide-induced p11 expression in human bronchial epithelial cells is mediated at least in part through increased binding of activator protein one to the p11 promoter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 15:49:09