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Titolo:
Immobilized-metal-ion affinity chromatography as a tool for the qualitative study of pepsinogen phosphorylation
Autore:
Kucerova, Z; Majercakova, P;
Indirizzi:
Charles Univ, Inst Pathophysiol, Fac Med 1, Prague 12853 2, Czech RepublicCharles Univ Prague Czech Republic 12853 2 rague 12853 2, Czech Republic
Titolo Testata:
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
fascicolo: 1-3, volume: 49, anno: 2001,
pagine: 523 - 531
SICI:
0165-022X(20011030)49:1-3<523:IACAAT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; CAPILLARY ZONE ELECTROPHORESIS;
Keywords:
immobilized-metal-ion affinity chromatography; ferric ions; pepsinogen; phosphoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Kucerova, Z Charles Univ, Inst Pathophysiol, Fac Med 1, Nemocnice 5, Prague 12853 2, Czech Republic Charles Univ Nemocnice 5 Prague Czech Republic 12853 2 public
Citazione:
Z. Kucerova e P. Majercakova, "Immobilized-metal-ion affinity chromatography as a tool for the qualitative study of pepsinogen phosphorylation", J BIOCH BIO, 49(1-3), 2001, pp. 523-531

Abstract

Affinity chromatography on immobilized Fe3+ ions-immobilized-metal-ion affinity chromatography (IMAC) method-was used for the determination of pepsinand pepsinogen phosphorylation. IMAC is a very powerful method for detailed studies of proteins. Dephosphorylation of the pepsinogens and pepsins hasno effect on their proteolytic ability. For this reason, the determinationof proteolytic activity was used for the detection of pepsinogen (pepsin) presence in the collected fractions as a very suitable and specific method. Pepsins and their zymogens probably have the same amounts of phosphate ions in their molecule. The exact definition of conditions is very important for the prepurification of the proteinases and for their analysis. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:21:13