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Titolo:
Time course of myocardial sodium accumulation after burn trauma: a P-31- and Na-23-NMR study
Autore:
Sikes, PJ; Zhao, PY; Maass, DL; Horton, JW;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Surg, Dallas, TX 75390 USA Univ Texas DallasTX USA 75390 W Med Ctr, Dept Surg, Dallas, TX 75390 USA Univ Texas, SW Med Ctr, Dept Radiol, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Med Ctr, Dept Radiol, Dallas, TX 75390 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 6, volume: 91, anno: 2001,
pagine: 2695 - 2702
SICI:
8750-7587(200112)91:6<2695:TCOMSA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT VENTRICULAR MYOCYTES; NA+-H+ EXCHANGE; THERMAL-INJURY; REPERFUSION INJURY; CELLULAR-ENERGY; HEART; ACIDOSIS; CALCIUM; PH; INHIBITION;
Keywords:
nuclear magnetic resonance spectroscopy; cardiomyocyte loading of the fluorescent indicator sodium-binding benzofuran; isophthalate; ventricular contraction-relaxation assessed; by Langendorff perfusion; myocardial acidosis (intracellular pH); myocardial high-energy phosphate stores;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Horton, JW Univ Texas, SW Med Ctr, Dept Surg, 5323 Harry Hines Blvd, Dallas, TX 75390USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75390 X 75390USA
Citazione:
P.J. Sikes et al., "Time course of myocardial sodium accumulation after burn trauma: a P-31- and Na-23-NMR study", J APP PHYSL, 91(6), 2001, pp. 2695-2702

Abstract

In this study, Na-23- and P-31- nuclear magnetic resonance (NMR) spectra were examined in perfused rat hearts harvested 1, 2, 4, and 24 h after 40% total body surface area burn trauma and lactated Ringer resuscitation, 4 ml.kg(-1).%(-1) burn. Na-23-NMR spectroscopy monitored myocardial intracellular Na+ using the paramagnetic shift reagent thulium 1,4,7,10-tetraazacyclododecane1,4,7,10-tetra(methylenephosphonic acid). Left ventricular function, cardiac high-energy phosphates (ATP/PCr), and myocyte intracellular pH werestudied by using P-31 NMR spectroscopy to examine the hypothesis that burn-mediated acidification of cardiomyocytes contributes to subsequent Na+ accumulation by this cell population. Intracellular Na+ accumulation was confirmed by sodium-binding benzofuran isophthalate loading and fluorescence spectroscopy in cardiomyocytes isolated 1, 2, 4, 8, 12, 18, and 24 h postburn. This myocyte Na+ accumulation as early as 2 h postburn occurred despite nochanges in cardiac ATP/PCr and intracellular pH. Left ventricular functionprogressively decreased after burn trauma. Cardiomyocyte Na+ accumulation paralleled cardiac contractile dysfunction, suggesting that myocardial Na+ overload contributes, in part, to the progressive postburn decrease in ventricular performance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 08:02:34