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Titolo:
Gender influences coronary L-type Ca2+ current and adaptation to exercise training in miniature swine
Autore:
Bowles, DK;
Indirizzi:
Univ Missouri, Coll Vet Med, Dept Vet Biomed Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 et Biomed Sci, Columbia, MO 65211 USA Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 ovasc Res Ctr, Columbia, MO 65211 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 6, volume: 91, anno: 2001,
pagine: 2503 - 2510
SICI:
8750-7587(200112)91:6<2503:GICLCC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; SARCOPLASMIC-RETICULUM CALCIUM; CARDIOVASCULAR-DISEASE; CHANNEL; RAT; ARTERIES; EXPRESSION; PORCINE; RELEASE;
Keywords:
electrophysiology; vascular smooth muscle; microcirculation; voltage-gated calcium channels;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Bowles, DK Univ Missouri, Coll Vet Med, Dept Vet Biomed Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 ci, Columbia, MO 65211 USA
Citazione:
D.K. Bowles, "Gender influences coronary L-type Ca2+ current and adaptation to exercise training in miniature swine", J APP PHYSL, 91(6), 2001, pp. 2503-2510

Abstract

Endurance exercise training increases smooth muscle L-type Ca2+ current density in both resistance and proximal coronary arteries of female miniatureswine. The purpose of the present study was to determine 1) whether genderdifferences exist in coronary smooth muscle (CSM) L-type Ca2+ current; density and 2) whether endurance training in males would demonstrate a similaradaptive response as females. Proximal, conduit (similar to1.0 mm), and resistance [similar to 200 mum (internal diameter)] coronary arteries were obtained from sedentary and treadmill-trained swine of both sexes. CSM were isolated by enzymatic digestion (collagenase plus elastase), and voltage-gated Ca2+-channel current (I-Ca) was determined by using whole cell voltage clamp during superfusion with 75 mM tetraethylammonium chloride and 10 mM BaCl2. Current-voltage relationships were obtained at test potentials from -60 to 70 mV from a holding potential of -80 mV, and I-Ca was normalized to cell capacitance (pA/pF). Endurance treadmill training resulted in similar increases in heart weight-to-body weight ratio, endurance time, and skeletalmuscle citrate synthase activity in male and female swine. I-Ca. density was significantly greater in males compared with females in both conduit (- 7.57 +/- 0.58 vs. - 4.14 +/- 0.47 pA/pF) and resistance arteries (-11.25 +/- 0.74 vs. -6.49 +/- 0.87 pA/pF, respectively). In addition, voltage-dependent activation of I-Ca in resistance arteries was shifted to more negative membrane potentials in males. Exercise training significantly increased I-Ca density in both conduit and resistance arteries in females (-7.01 +/- 0.47 and -9.73 +/- 1.13 pA/pF, respectively) but had no effect in males (-8.61+/- 0.50 and -12.04 +/- 1.07 pA/pF, respectively). Thus gender plays a significant role in determining both the magnitude and voltage dependence of I-Ca in CSM and the adaptive response of I-Ca to endurance training.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:04:30