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Titolo:
Simultaneous determination of losartan and hydrochlorothiazide in combineddosage forms by first-derivative spectroscopy and high-performance thin-layer chromatography
Autore:
Shah, SA; Rathod, IS; Suhagia, BN; Savale, SS; Patel, JB;
Indirizzi:
Lallubhai Motilal Coll Pharm, Dept Qual Assurance, Ahmedabad 380009, Gujarat, India Lallubhai Motilal Coll Pharm Ahmedabad Gujarat India 380009 ujarat, India
Titolo Testata:
JOURNAL OF AOAC INTERNATIONAL
fascicolo: 6, volume: 84, anno: 2001,
pagine: 1715 - 1723
SICI:
1060-3271(200111/12)84:6<1715:SDOLAH>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVE ANTIHYPERTENSIVE AGENT; II RECEPTOR ANTAGONISTS; LIQUID-CHROMATOGRAPHY; COMBINED FORMULATIONS; COZAAR(R) TABLETS; SPECTROPHOTOMETRY; DUP-753; PHARMACOLOGY; METABOLITE; POTASSIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Shah, SA Lallubhai Motilal Coll Pharm, Dept Qual Assurance, Ahmedabad 380009, Gujarat, India Lallubhai Motilal Coll Pharm Ahmedabad Gujarat India 380009 ndia
Citazione:
S.A. Shah et al., "Simultaneous determination of losartan and hydrochlorothiazide in combineddosage forms by first-derivative spectroscopy and high-performance thin-layer chromatography", J AOAC INT, 84(6), 2001, pp. 1715-1723

Abstract

Losartan (LST) is the first orally active nonpeptide angiotensin-II receptor antagonist with an improved safety and tolerability profile. It is prescribed alone or in combination with hydrochlorothiazide (HCTZ) for the treatment of moderate-to-severe hypertension. This paper describes the development of 2 methods that use different techniques, first-derivative spectroscopy and high-performance thin-layer chromatography (HPTLC), to determine LST and HCTZ in the presence of each other. LST and HCTZ in combined preparations were quantitated by using the first-derivative responses at 271.6 nm forLST and 335.0 nm for HCTZ in spectra of their solutions in water. The linearity ranges are 30-70 mug/mL for LST and 7.5-17.5 mug/mL for HCTZ with correlation coefficients of 0.9998 and 0.9997, respectively. In the HPTLC method, a mobile phase of chloroform-methanol-acetone-formic acid (7.5 + 1.5 + 0.5 + 0.03, v/v) and a prewashed Silica Gel G60 F-254 TLC plate as the stationary phase were used to resolve LST and HCTZ in a mixture. Two well-separated and sharp peaks for LST and HCTZ were obtained at Rf values of 0.61 +/- 0.02 and 0.41 +/- 0.02, respectively. LST and HCTZ were quantitated at 254.0 nm. The linearity ranges obtained for the HPTLC method are 400-1200 and100-300 ng/spot with corresponding correlation coefficients of 0.9944 and 0.9979, for LST and HCTZ, respectively. Both methods were validated, and the results were compared statistically. They were found to be accurate, specific, and reproducible. The methods were successfully applied to the estimation of LST and HCTZ in combined tablet formulations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:52:22