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Titolo:
Determination of cellular carbohydrates in peanut fungal pathogens and baker's yeast by capillary electrophoresis and electrochromatography
Autore:
Zhang, MQ; Melouk, HA; Chenault, K; El Rassi, Z;
Indirizzi:
Oklahoma State Univ, Dept Chem, USDA, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 hol, Stillwater, OK 74078 USA Oklahoma State Univ, USDA ARS, Dept Entomol & Plant Pathol, Stillwater, OK74078 USA Oklahoma State Univ Stillwater OK USA 74078 thol, Stillwater, OK74078 USA
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 11, volume: 49, anno: 2001,
pagine: 5265 - 5269
SICI:
0021-8561(200111)49:11<5265:DOCCIP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
OCTADECYL-SULFONATED-SILICA; STATIONARY PHASES; RETENTION BEHAVIOR; BETA-1,3-GLUCANASE; ACIDS;
Keywords:
capillary electrophoresis; capillary electrochromatography; cellular carbohydrates; Sclerotinia minor; Sclerotium rolfsii; baker's yeast; fungal pathogens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: El Rassi, Z Oklahoma State Univ, Dept Chem, USDA, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 ter, OK 74078 USA
Citazione:
M.Q. Zhang et al., "Determination of cellular carbohydrates in peanut fungal pathogens and baker's yeast by capillary electrophoresis and electrochromatography", J AGR FOOD, 49(11), 2001, pp. 5265-5269

Abstract

In this work, the quantitation of cellular carbohydrates, namely chitin and glucan, in peanut fungal pathogens and baker's yeast was carried out by capillary electrophoresis (CE) and capillary electrochromatography (CEC). The chitin and glucan of the fungi were hydrolyzed by the enzymes chitinase and glucanase, respectively, to their corresponding sugar monomers N-acetylglucosamine (GlcNAc) and glucose (Glc). These two monosaccharides were then tagged with 6-aminoquinoline (6-AQ) to allow their separation and detectionin CE and CEC. The 6-AQ derivatives of GlcNAc and Glc formed the basis forthe determination by CE and CEO of chitin and glucan in peanut fungi and baker's yeast. Several parameters affecting the separation of the 6-AQ derivatives of GlcNAc and Glc, including the separation voltage and the composition of the running electrolyte, were investigated. Under the optimized separation conditions, the contents of cellular carbohydrates including N-acetylglucosamine, chitin, glucose, and glucan in some fungi, such as Sclerotinia minor, Sclerotium rolfsii, and baker's yeast, were successfully determined. The method described here allowed the assessment of genetic differences in Sclerotium rolfsii isolates from various locations.

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Documento generato il 05/04/20 alle ore 07:54:06