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Titolo:
Conformation changes in brain calcineurin in diabetic rats with or withouttreatment with vanadyl sulfate
Autore:
Li, H; Wei, Q;
Indirizzi:
Beijing Normal Univ, Dept Biochem & Mol Biol, Inst Life Sci, Beijing 100875, Peoples R China Beijing Normal Univ Beijing Peoples R China 100875 0875, Peoples R China
Titolo Testata:
IUBMB LIFE
fascicolo: 6, volume: 51, anno: 2001,
pagine: 373 - 376
SICI:
1521-6543(200106)51:6<373:CCIBCI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATASE; CALMODULIN; INACTIVATION; BINDING; COMPLEX; ION;
Keywords:
calcineurin; conformation; diabetes; electron spin resonance; vanadyl sulfate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Wei, Q Beijing Normal Univ, Dept Biochem & Mol Biol, Inst Life Sci, Beijing 100875, Peoples R China Beijing Normal Univ Beijing Peoples R China 100875 eoples R China
Citazione:
H. Li e Q. Wei, "Conformation changes in brain calcineurin in diabetic rats with or withouttreatment with vanadyl sulfate", IUBMB LIFE, 51(6), 2001, pp. 373-376

Abstract

Recent studies have shown that vanadium salts are able to reduce blood glucose in diabetics and overcome, to some degree, insulin resistance. This paradigm has been followed to monitor the effects of diabetes and vanadyl treatment on brain calcineurin (CN), an important protein phosphatase. Male rats were rendered diabetic by a single injection of streptozotocin (STZ), resulting in an elevation of blood glucose from 108 +/- 13 to >400 mg/dl. Diabetic animals were given vanadyl sulfate trihydrate (0.5 mg/dl.) in their drinking water for 3 weeks, which led to a fall in blood glucose to 156 +/- 53 mg/ml. Brain CN activity (units/mg brain protein) in diabetic rats was 77% that of control animals, whereas vanadyl-treated diabetic animals were characterized by CN activities like that of controls. CN was purified from brains of control animals, STZ-induced diabetic animals, and STZ-induced diabetic animals receiving vanadyl, then spin-labeled with 3-maleimide-proxyl and studied via electron spin resonance spectroscopy. The rotational correlation time of CN from control animals and vanadyl-treated diabetic animals was 6.4 x 10(-11) s(-1), whereas that from STZ-induced-diabetic animals was8 x 10(-11) s(-1). Thus, STZ-induced diabetes in rats results in an increase in the rotational correlation time of brain CN relative to control animals, yet vanadyl treatment of STZ-induced diabetic animals reduced the rotational correlation time to that of control. These data suggest that diabetescan lead to apparent conformational changes in brain CN; also, CN conformation in diabetic rats was restored by vanadyl treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:03:24