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Titolo:
Stage-specific expression of Gadd45 induced by X-irradiation in rat spermatogenesis
Autore:
West, A; Priante, G; Lahdetie, J;
Indirizzi:
Univ Turku, Dept Med Genet, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 t Med Genet, FIN-20520 Turku, Finland Turku Grad Sch Biomed Sci, Turku, Finland Turku Grad Sch Biomed Sci Turku Finland Sch Biomed Sci, Turku, Finland
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 1, volume: 78, anno: 2002,
pagine: 29 - 39
SICI:
0955-3002(200201)78:1<29:SEOGIB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE CHECKPOINT; WILD-TYPE P53; DNA-DAMAGE; TOPOISOMERASE-II; SEMINIFEROUS EPITHELIUM; HUMAN FIBROBLASTS; NUCLEAR ANTIGEN; MUSCLE-CELLS; G(1) ARREST; MALE-MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: West, A Univ Turku, Dept Med Genet, Kiinamyllynkatu 10, FIN-20520 Turku, Finland Univ Turku Kiinamyllynkatu 10 Turku Finland FIN-20520 ku, Finland
Citazione:
A. West et al., "Stage-specific expression of Gadd45 induced by X-irradiation in rat spermatogenesis", INT J RAD B, 78(1), 2002, pp. 29-39

Abstract

Purpose: Gadd45 is involved in the response to DNA damage in somatic cells. The effect of X-irradiation and chemical treatments on expression of Gadd45 and two other 53-regulated genes, p21 and cyclin-G, was studied in rat testis. Materials and methods: The reverse-transcriptase polymerase chain reaction(RT-PCR) was performed on testis extracts of control, X-irradiated (6 Gy),etoposide (10 mg kg(-1)) and adriamycin (5 mg kg(-1))-treated rats. For stage-specific analysis, seminiferous tubules were isolated and segments representing the 14 epithelial stages were obtained. Results: In whole testis extracts, increases in Gadd45, p21 and cyclin-G expression were detectable after irradiation, but not after etoposide or adriamycin treatments. Analysis of fractions consisting of defined epithelial stages showed a high expression of Gadd45 in stages VII-XII and of p21 in stages VII-VIII. Irradiation significantly increased the level of Gadd45 mRNA in stages VI-VIII and of p21 mRNA in stages VI-I. Although no overall increase could be observed in whole testis samples of the etoposide-treated rat, stage-specific analysis revealed an induction of p21 expression in stages XIII-I. Gadd45 and cyclin-G mRNA were localized to spermatocytes and round spermatids known to express p21. Conclusions : Although X-irradiation, etoposide and adriamycin are known spermatogenic mutagens and activators of apoptosis, only X-rays induce slightly Gadd45 expression in testis. This small induction was very stage specific.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:40:49