Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
UGA4 gene encoding the gamma-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
Autore:
Moretti, MB; Batlle, A; Garcia, SC;
Indirizzi:
Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, CIPYP, RA-1428 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1428 Aires, DF, Argentina
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 12, volume: 33, anno: 2001,
pagine: 1202 - 1207
SICI:
1357-2725(200112)33:12<1202:UGETGA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FINGER TRANSCRIPTION FACTOR; AMBIENT PH; ASPERGILLUS-NIDULANS; BINDING-SITES; YEAST; CLUSTER; ACTIVATION; PROMOTER; DEPENDS; GROWTH;
Keywords:
delta-aminolevulinic acid; gamma-aminobutyric acid; UGA4 gene; Saccharomyces cerevisiae; ambient pH; transcriptional regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Batlle, A Viamonte 1881,10o A,C1056ABA, Buenos Aires, DF, Argentina Viamonte 1881,10o A,C1056ABA Buenos Aires DF Argentina gentina
Citazione:
M.B. Moretti et al., "UGA4 gene encoding the gamma-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene", INT J BIO C, 33(12), 2001, pp. 1202-1207

Abstract

Background and aims: biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH responseof UGA4 gene encoding delta -aminolevulinic acid and gamma -aminobutyric acid permease in Saccharomyces cerevisiae. Methods: we analyzed the effect of pH on the expression of UGA4 gene measuring beta -galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes. which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4transcription is dependent on pH. Using a strain lacking Uga43p we clearlyshowed that the effect of ambient pH on UGA4 expression is not a secondaryeffect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: here. we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediatedby Rim101p through the consensus site 5'-GCCARG-3' at 237 bp preceding theUGA4 coding sequence (201). (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 14:06:24