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Titolo:
Two novel insect defensins from larvae of the cupreous chafer, Anomala cuprea: purification, amino acid sequences and antibacterial activity
Autore:
Yamauchi, H;
Indirizzi:
Forestry & Forest Prod Res Inst, Dept Forest Entomol, Insect Management Lab, Tsukuba, Ibaraki 3058687, Japan Forestry & Forest Prod Res Inst TsukubaIbaraki Japan 3058687 8687, Japan
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 32, anno: 2001,
pagine: 75 - 84
SICI:
0965-1748(200112)32:1<75:TNIDFL>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEREGRINA FLESH-FLY; SARCOPHAGA-PEREGRINA; MOLECULAR-CLONING; ANTIMICROBIAL PEPTIDES; AEDES-AEGYPTI; STEINERNEMA-KUSHIDAI; TENEBRIO-MOLITOR; INNATE IMMUNITY; VECTOR MOSQUITO; SAPECIN-B;
Keywords:
amino acid sequence; insect defensin; antibacterial activity; innate immunity; Anomala cuprea; Xenorhabdus japonicus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Yamauchi, H Forestry & Forest Prod Res Inst, Dept Forest Entomol, Insect Management Lab, POB 16, Tsukuba, Ibaraki 3058687, Japan Forestry & Forest Prod Res Inst POB 16 Tsukuba Ibaraki Japan 3058687
Citazione:
H. Yamauchi, "Two novel insect defensins from larvae of the cupreous chafer, Anomala cuprea: purification, amino acid sequences and antibacterial activity", INSEC BIO M, 32(1), 2001, pp. 75-84

Abstract

A humoral immune response in larvae of the coleopteran insect, Anomala cuprea has been examined for exploring the molecular basis of host-pathogen interactions. The antibacterial activity against the Gram-positive strain. Micrococcus luteus was detected at a low level in absence of injection. The activity increased strikingly in the hemolymph of the larvae challenged withEscherichia coli, showing the fluctuating profile through a time course, which consists of the static induction phase, the production phase rising toa maximum level, and the reduction phase extending over a long duration. Two peptides were purified and characterized by reverse-phase HPLC, Edman degradation and mass spectrometry. They were isoforms, composed of similar sequences with two amino acid substitutions in 43 residues, and novel membersof the insect defensins, cysteine-rich antibacterial peptides. Anomala defensins A and B showed potent activity against Gram-positive bacteria, with slight differences in activity against a few strains of tested bacteria. Anomala defensin B was active at high concentration of 40 mum against the Gram-negative strain, Xenorhabdus japonicus, a pathogen toward the host, A. cuprea larvae. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:49:04