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Titolo:
High resolution H-1 NMR spectroscopic studies of the composition of the haemolymph of crowd- and solitary-reared nymphs of the desert locust, Schistocerca gregaria
Autore:
Lenz, EM; Hagele, BF; Wilson, ID; Simpson, SJ;
Indirizzi:
Univ Keele, Dept Chem, Keele ST5 5BG, Staffs, England Univ Keele Keele Staffs England ST5 5BG m, Keele ST5 5BG, Staffs, England Univ Oxford, Dept Zool, Oxford OX1 3PS, England Univ Oxford Oxford England OX1 3PS d, Dept Zool, Oxford OX1 3PS, England Univ Oxford, Univ Museum, Oxford OX1 3PS, England Univ Oxford Oxford England OX1 3PS Univ Museum, Oxford OX1 3PS, England
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 32, anno: 2001,
pagine: 51 - 56
SICI:
0965-1748(200112)32:1<51:HRHNSS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; BIOLOGICAL-FLUIDS; JUVENILE-HORMONE; PHASE; POLYAMINE; ASSIGNMENT; BEHAVIOR; CRICKETS;
Keywords:
NMR; haemolymph metabolites; Schistocerca gregaria; phase polyphenism; locust; putrescine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Wilson, ID AstraZeneca Pharmaceut, Dept Drug Metab & Phamacokinet, Alderley Pk, Macclesfield SK10 4TG, Cheshire, England AstraZeneca Pharmaceut Alderley Pk Macclesfield Cheshire England SK10 4TG
Citazione:
E.M. Lenz et al., "High resolution H-1 NMR spectroscopic studies of the composition of the haemolymph of crowd- and solitary-reared nymphs of the desert locust, Schistocerca gregaria", INSEC BIO M, 32(1), 2001, pp. 51-56

Abstract

High resolution H-1 NMR spectroscopy provided a rapid and non-invasive means of establishing a multicomponent profile of the low molecular weight organic metabolites present in the haemolymph of crowd-reared and solitary-reared desert locusts. Assignments of the H-1 NMR resonances detected in the one-dimensional (ID) spectra were supported by the comparison of chemical shifts with those of commonly found metabolites, the application of 2D NMR spectroscopy, and spiking of authentic standards into the sample. The haemolymph metabolite profile was found to comprise trehalose, a series of amino acids, organic acids, lipids. ethanol and the polyamine putrescine. In total, it was possible to detect simultaneously and potentially quantify over 20endogenous compounds. An interesting difference between the phases was thehigher putrescine concentrations present in solitary-reared nymphs. which may indicate a role of this compound in phase determination. In addition, differences in the concentrations of trehalose. lipids, acetate and ethanol were also observed between solitary-reared and gregarious-reared nymphs enabling the ready differentiation of the two phases based on the NMR spectra of the haemolymph. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 17:38:41