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Titolo:
Peripheral astrocyte processes: Monitoring by selective immunostaining forthe actin-binding ERM proteins
Autore:
Derouiche, A; Frotscher, M;
Indirizzi:
Univ Dresden, Inst Anat, D-01307 Dresden, Germany Univ Dresden Dresden Germany D-01307 Inst Anat, D-01307 Dresden, Germany Max Planck Inst Brain Res, D-6000 Frankfurt, Germany Max Planck Inst BrainRes Frankfurt Germany D-6000 00 Frankfurt, Germany Univ Freiburg, Inst Anat, D-79001 Freiburg, Germany Univ Freiburg Freiburg Germany D-79001 t Anat, D-79001 Freiburg, Germany
Titolo Testata:
GLIA
fascicolo: 3, volume: 36, anno: 2001,
pagine: 330 - 341
SICI:
0894-1491(200112)36:3<330:PAPMBS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLARY ACIDIC PROTEIN; GLUTAMINE-SYNTHETASE; EZRIN IMMUNOREACTIVITY; SYNAPTIC PLASTICITY; RECEPTOR ACTIVATION; EPITHELIAL-CELLS; FAMILY MEMBERS; GLIAL-CELLS; LOCALIZATION; CYTOSKELETON;
Keywords:
glia; ezrin; radixin; moesin; actin cytoskeleton; filopodia; rat hippocampus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Derouiche, A Univ Dresden, Inst Anat, Fetscherstr 74, D-01307 Dresden, Germany Univ Dresden Fetscherstr 74 Dresden Germany D-01307 Germany
Citazione:
A. Derouiche e M. Frotscher, "Peripheral astrocyte processes: Monitoring by selective immunostaining forthe actin-binding ERM proteins", GLIA, 36(3), 2001, pp. 330-341

Abstract

Astrocytes extend thin lamellate processes in the neuropil, in particular around synapses, where they can modulate synaptic function or mediate glial-neuronal communication. Previous studies have shown that these lamellate perisynaptic processes change their shape in response to neuronal activity, but the underlying mechanisms have remained unclear. Similarly, the molecular composition of these fine, sheetlike astrocytic processes (often 50-100 nm wide) is not understood but has to be related to their dynamic properties. To this end, we have studied the presence of ezrin, radixin, and moesin (ERM proteins) in the rat hippocampus and in primary cultured astrocytes, applying immunoperoxidase, immunofluorescence, and immunogold techniques. These three ERM proteins are known as actin-binding proteins that link the cell membrane to the actin cytoskeleton, particularly in microvillus-bearing epithelial cells. In cell culture, anti-ezrin and antiradixin, but not antimoesin, antibodies were specific for astrocytes, which often displayed selective staining of filopodia and microvilli. Nonoverlapping visualization ofastrocytic peripheral and stem processes was obtained by immunocytochemical double labeling for ezrin and GFAP, respectively. In sections of rat hippocampus, homogeneous labeling of the neuropil, but not of cell layers, resulted from immunostaining of fine, peripheral astrocyte processes, as confirmed ultrastructurally. Our data show that the fine peripheral processes of astrocytes, which also constitute the perisynaptic glial sheath, are specialized in that they contain characteristic actin-associated molecules, likely to contribute to their dynamic properties. Applying antiezrin and anti-radixin as selective markers, plasticity of these perisynaptic glial processes can be analyzed. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:08:17