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Titolo:
Requirement of functional ryanodine receptor type 3 for astrocyte migration
Autore:
Matyash, M; Matyash, V; Nolte, C; Sorrentino, V; Kettenmann, H;
Indirizzi:
Max Delbruck Ctr Mol Med, D-13092 Berlin, Germany Max Delbruck Ctr Mol Med Berlin Germany D-13092 D-13092 Berlin, Germany Univ Siena, Dept Neurosci, Mol Med Sect, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 rosci, Mol Med Sect, I-53100 Siena, Italy San Raffaele Sci Inst, I-20132 Milan, Italy San Raffaele Sci Inst Milan Italy I-20132 Sci Inst, I-20132 Milan, Italy
Titolo Testata:
FASEB JOURNAL
fascicolo: 13, volume: 15, anno: 2001,
pagine: NIL_279 - NIL_303
SICI:
0892-6638(200111)15:13<NIL_279:ROFRRT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE SKELETAL-MUSCLE; CA2+ RELEASE CHANNELS; INTRACELLULAR CALCIUM; IN-VITRO; DIFFERENTIAL DISTRIBUTION; ENDOGENOUS EFFECTORS; ACTIN CYTOSKELETON; G-PROTEIN; CONTRACTION; CELLS;
Keywords:
RyR3 knockout mice; 4-chloro-m-cresol; in vitro wound-healing assay; cell migration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Kettenmann, H Max Delbruck Ctr Mol Med, Robert Rossle Str 10, D-13092 Berlin, Germany Max Delbruck Ctr Mol Med Robert Rossle Str 10 Berlin Germany D-13092
Citazione:
M. Matyash et al., "Requirement of functional ryanodine receptor type 3 for astrocyte migration", FASEB J, 15(13), 2001, pp. NIL_279-NIL_303

Abstract

Astrocyte motility plays an important role in the response of the brain toinjury and during regeneration. We used two in vitro assays, a wound-healing model and a chemotaxis assay, to study mechanisms that control astrocytemotility. Ryanodine receptors (RyR), intracellular calcium-release channels, modulate intracellular Ca2+ levels, and also motility: 1) blocking RyR with antagonizing concentration of ryanodine (200 muM) strongly attenuated motility and 2) motility of astrocytes cultured from homozygous RyR type 3 knockout mice was impaired strongly compared with wild-type. In contrast, MIP-1 alpha -induced chemotaxis was neither impaired in the presence of ryanodine nor in the cells from the knockout animals. Reverse transcription-polymerase chain reaction (RT-PCR) analysis combined with Western blotting and immunocytochemistry confirmed the expression of RyR type 3, but not type 1 or 2 in cultured and acutely isolated astrocytes. RyR in astrocytes are linked to Ca2+ signaling because the RyR agonist 4-chloro-m-cresol induced a release of Ca2+ from intracellular stores. These results indicate that astrocytes express only RyR type 3 and that this receptor is important for controlling astrocyte motility.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:14:56