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Titolo:
Influence of alpha-melanocyte-stimulating hormone and of ultraviolet radiation on the transfer of melanosomes to keratinocytes
Autore:
Virador, VM; Muller, J; Wu, XF; Abdel-Malek, ZA; Yu, ZX; Ferrans, VJ; Kobayashi, N; Wakamatsu, K; Ito, S; Hammer, JA; Hearing, VJ;
Indirizzi:
NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA US FDA, Ctr Biol Evaluat & Res, Div Viral Prod, Bethesda, MD 20892 USA US FDA Bethesda MD USA 20892 Res, Div Viral Prod, Bethesda, MD 20892 USA NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 I, Cell Biol Lab, NIH, Bethesda, MD 20892 USA Univ Cincinnati, Dept Dermatol, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 ermatol, Cincinnati, OH 45267 USA NHLBI, Pathol Sect, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892NHLBI, Pathol Sect, Bethesda, MD 20892 USA Nara Med Univ, Dept Dermatol, Kashihara, Nara 634, Japan Nara Med Univ Kashihara Nara Japan 634 rmatol, Kashihara, Nara 634, Japan Fujita Hlth Univ, Sch Hlth Sci, Dept Chem, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Toyoake Aichi Japan 4701192 oyoake, Aichi 4701192, Japan
Titolo Testata:
FASEB JOURNAL
fascicolo: 13, volume: 15, anno: 2001,
pagine: NIL_135 - NIL_161
SICI:
0892-6638(200111)15:13<NIL_135:IOAHAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MELANOCYTES; EPIDERMAL-KERATINOCYTES; IN-VITRO; PROTEIN; CELLS; EXOCYTOSIS; SYNTAXIN-4; TRANSPORT; SECRETION; SNAP-23;
Keywords:
skin color; tanning; melanosome transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Hearing, VJ NCI, Cell Biol Lab, NIH, Bldg 37,Room 1B25, Bethesda, MD 20892USA NCI Bldg 37,Room 1B25 Bethesda MD USA 20892 esda, MD 20892 USA
Citazione:
V.M. Virador et al., "Influence of alpha-melanocyte-stimulating hormone and of ultraviolet radiation on the transfer of melanosomes to keratinocytes", FASEB J, 15(13), 2001, pp. NIL_135-NIL_161

Abstract

The epidermal melanin unit in human skin is composed of melanocytes and keratinocytes. Melanocytes, located in the basal layer of the epidermis, manufacture melanin-loaded organelles called melanosomes. Through their dendritic processes, melanocytes distribute melanosomes to neighboring keratinocytes, where their presence confers to the skin its characteristic color and photoprotective properties. In this study, we used murine melanocytes and keratinocytes alone and in coculture to characterize the processes involved in melanosome transfer. Ultraviolet (UV) radiation induced an accumulation of melanosomes in melanocytes, whereas treatment with alpha -melanocyte-stimulating hormone (MSH) induced exocytosis of melanosomes accompanied by ruffling of the melanocyte membrane. We found that keratinocytes phagocytose melanosomes and latex beads equally well and that this phagocytic process wasincreased by exposure of keratinocytes to UV radiation or to MSH. Coculture of melanocytes and keratinocytes resulted in an increase in MSH released to the medium. Gene array analysis of MSH-treated melanocytes showed up-regulation of many genes associated with exocytosis. In our studies, we never observed cytophagocytosis of melanosome-filled processes. This result, together with the other findings, suggests that a combination of signals that increase melanosome production and release by melanocytes and that stimulatephagocytosis by keratinocytes are the most relevant mechanisms involved inskin tanning.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 08:07:31