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Titolo:
Nerve growth factor in combination with second messenger analogues causes neuronal differentiation of PC12 cells expressing a dominant inhibitory Rasprotein without inducing activation of extracellular signal-regulated kinases
Autore:
Boglari, G; Szeberenyi, J;
Indirizzi:
Univ Pecs, Sch Med, Dept Med Biol, H-7624 Pecs, Hungary Univ Pecs Pecs Hungary H-7624 h Med, Dept Med Biol, H-7624 Pecs, Hungary
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 14, anno: 2001,
pagine: 1445 - 1454
SICI:
0953-816X(200111)14:9<1445:NGFICW>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASE; NEURITE OUTGROWTH; MAP KINASE; CYCLIC-AMP; SUSTAINED ACTIVATION; DEPENDENT TRANSCRIPTION; PHEOCHROMOCYTOMA CELLS; NUCLEAR TRANSLOCATION; GENE-EXPRESSION; SODIUM-CHANNELS;
Keywords:
dibutyryl cyclic AMP; Ha-Ras Asn17; ionomycin; Trk;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Szeberenyi, J Univ Pecs, Sch Med, Dept Med Biol, Szigeti 12, H-7624 Pecs, Hungary Univ Pecs Szigeti 12 Pecs Hungary H-7624 7624 Pecs, Hungary
Citazione:
G. Boglari e J. Szeberenyi, "Nerve growth factor in combination with second messenger analogues causes neuronal differentiation of PC12 cells expressing a dominant inhibitory Rasprotein without inducing activation of extracellular signal-regulated kinases", EUR J NEURO, 14(9), 2001, pp. 1445-1454

Abstract

In the present work, nerve growth factor (NGF) was used in combination with the calcium ionophore, ionomycin or dibutyryl cyclic AMP (dbcAMP), to study the connection between neuronal differentiation and extracellular signal-regulated kinase (ERK) activation of PC12 rat pheochromocytoma cells expressing a dominant negative, Ha-Ras Asn17 protein. Due to the block of endogenous Ras activity, neurite outgrowth in response to NGF is completely inhibited in these cells. However, this blockade can be bypassed by combined treatment with NGF plus ionomycin or NGF plus dbcAMP. The mitogen-activated protein kinase (MAPK)/ERK kinase inhibitor, PD98069, proved to be insufficient in inhibiting the neurite outgrowth under these conditions. Moreover, although both long-term ERK activation and nuclear translocation of ERKs are believed to be key events in neuronal differentiation, neither detectable ERK phosphorylation, nor nuclear translocation of these enzymes, occurred upon combination treatments in our experimental system. However, the neuritogenesis induced by either the combination of NGF/ionomycin or NGF/dbcAMP was inhibited by the Trk inhibitor, K252a. Ras-independent pathways, originating from the NGF receptor, can thus synergize with second messenger analoguesbypassing the ERK cascade but leading to the same biological result-neurite formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:52:20