Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential effects of radiocontrast agents on human umbilical vein endothelial cells: Cytotoxicity and modulators of thrombogenicity
Autore:
Fauser, C; Ullisch, EV; Kubler, W; Haller, C;
Indirizzi:
Univ Heidelberg, Dept Med 3 Cardiol, Heidelberg, Germany Univ Heidelberg Heidelberg Germany t Med 3 Cardiol, Heidelberg, Germany
Titolo Testata:
EUROPEAN JOURNAL OF MEDICAL RESEARCH
fascicolo: 11, volume: 6, anno: 2001,
pagine: 465 - 472
SICI:
0949-2321(20011120)6:11<465:DEORAO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONIONIC CONTRAST-MEDIA; TRANSLUMINAL CORONARY ANGIOPLASTY; RANDOMIZED TRIAL; UNSTABLE ANGINA; THROMBOTIC COMPLICATIONS; PROTEIN-C; FACTOR-V; OSMOLALITY; INVITRO; NEPHROTOXICITY;
Keywords:
angiography; acute coronary syndrome; endothelial cell injury; coagulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Haller, C Med Univ Klin, Abt 3, Bergheimerstr 58, D-69115 Heidelberg, Germany Med Univ Klin Bergheimerstr 58 Heidelberg Germany D-69115 rmany
Citazione:
C. Fauser et al., "Differential effects of radiocontrast agents on human umbilical vein endothelial cells: Cytotoxicity and modulators of thrombogenicity", EUR J MED R, 6(11), 2001, pp. 465-472

Abstract

The endothelium plays a central role in the regulation of blood flow and coagulation. The impact of radiocontrast agents on endothelial cells is therefore potentially clinically important, particularly in percutaneous interventions for acute coronary thrombosis. The effects of radiocontrast agents on endothelial cell viability and determinants of thrombogenicity were studied in human umbilical vein endothelial cells (HUVECs) in vitro. Intercellular tight junctions were assessed using immunofluorescence microscopy and measurement of the transmonolayer electrical resistance (TMR). The concentrations of endothelin-1 (E), von Willebrand factor (vWF), plasminogen activator inhibitor-1 (PAI-1) and thrombomodulin (T) were measured in the cell culture media. The ionic, high osmolal radiocontrast agent diatrizoate inducedconcentration-dependent cell death and an opening of tight junctions with the attendant abolition of the TMR. The concentration of E decreased, vWF increased in the cell culture media, the concentration of PAI-1 and T was not significantly changed by diatrizoate. Radiocontrast agents with reduced osmolality (ioxaglate: ionic; iopamidol: non-ionic) induced an increase in PAI-1 and vWF, but E and T were not significantly changed. Conclusions: Radiocontrast agents have differential effects on endothelial cells in vitro including the secretion of modulators of thrombogenesis. The effects are mostpronounced in the markedly hyperosmolal compound diatrizoate suggesting a contributory role of hypertonicity. Ioxaglate and iopamidol both increased the prothrombotic factors vWF and PAI-1 to the same degree indicating a similar risk of thrombogenicity between low-osmolal ionic and non-ionic radiocontrast agents in this in vitro model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 08:45:49