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Titolo:
Histological characteristics of induced acute peptic injury in equine gastric squamous epithelium
Autore:
Murray, MJ; Eichorn, ES; Jeffrey, SC;
Indirizzi:
Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Marion duPont Scott Equine Med Ctr, Leesburg, VA 20177 USA Virginia PolytechInst & State Univ Leesburg VA USA 20177 g, VA 20177 USA
Titolo Testata:
EQUINE VETERINARY JOURNAL
fascicolo: 6, volume: 33, anno: 2001,
pagine: 554 - 560
SICI:
0425-1644(200111)33:6<554:HCOIAP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; THOROUGHBRED RACEHORSES; ULCERS; RANITIDINE; ULCERATION; ESOPHAGUS; LESIONS; HORSES; FOALS; RATS;
Keywords:
horse; acute peptic injury; gastric squamous epithelium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Murray, MJ Virginia Polytech Inst & State Univ, Virginia Maryland Reg CollVet Med, Marion duPont Scott Equine Med Ctr, Leesburg, VA 20177 USA Virginia Polytech Inst & State Univ Leesburg VA USA 20177 USA
Citazione:
M.J. Murray et al., "Histological characteristics of induced acute peptic injury in equine gastric squamous epithelium", EQUINE V J, 33(6), 2001, pp. 554-560

Abstract

The objective of the study reported here was to characterise the microscopic appearance of peptic-injured equine gastric squamous epithelium in relation to the duration of peptic injury. Erosions and ulcers were induced in equine gastric squamous epithelium using a feed deprivation protocol that results in prolonged increased gastric acidity. Specimens of normal gastric mucosa and mucosa with lesions created after 48 and 96 h of feed deprivationwere compared for characteristics associated with angiogenesis and mucosalproliferation. Fifteen mature horses, 9 geldings and 6 mares, age 3-20 years, were divided into 3 groups. Group 1 (n = 5) had normal-appearing gastric squamous mucosal epithelium and had been killed due to problems unrelatedto the gastrointestinal tract. Groups 2 (n = 5) and 3 (n = 5) had lesions induced in the gastric squamous epithelium by alternating 24 h periods of feed deprivation and ad libitum access to hay, for totals of 48 and 96 h feed deprivation, respectively. Following lethal injection of barbiturate, stomachs were removed and fixed by filling with 4-6 1 10% buffered formalin. Sections were made from lesions in the gastric squamous epithelium adjacent to the margo plicatus along the right side of the stomach/greater curvatureand the lesser curvature. Measurements of total epithelial thickness, keratinised epithelium, nonkeratinised epithelium, epithelial projections, capillary extension into the epithelium and lamina propria thickness were made. The cross-sectional areas of arterial and venous vascular structures in the lamina propria at the lesions and their margins were measured using imageanalysis software. All horses, except one, in Group 2 developed erosions or ulcers in the gastric squamous epithelium after feed deprivation. There were several changesin the epithelium adjacent to erosions and ulcers, compared to normal epithelium, from horses in Groups 2 and 3: total epithelial thickness was significantly (P <0.05) greater, including both keratinised and nonkeratinised layers in most specimens; the length of epithelial projections and extent towhich capillaries from the lamina propria extended toward the luminal surface, and the cross-sectional area of vascular structures (arterioles, capillaries, venules) in the lamina propria were significantly greater. Epithelial thickness of erosion beds was not significantly less than normal epithelium, although a greater proportion of the epithelium in erosions consisted of epithelial projections (Group 1. 23%; Group 2, 76%; Group 3, 72%). The cross-sectional area of vascular structures in the lamina propria beneath erosions was significantly greater than in normal mucosa only in Group 2 tissues, whereas in the lamina propria of ulcers it was significantly greater than in normal mucosa only in Group 3 tissues. The epithelial proliferation and increased vascular cross-sectional area in the lamina propria associated with peptic-induced gastric lesions are consistent with processes associated with the initiation of ulcer healing, andthese changes temporally coincided with the initiation of peptic insult tothe gastric squamous epithelium. These findings demonstrate that processesthat promote ulcer healing begin soon after peptic injury and that they progress even with repeated peptic injury. Furthermore, our findings support observations that gastric ulcers often heal without medical intervention, and the theory that medications that reduce gastric acidity do not initiate healing, but rather facilitate ulcer healing by providing a microenvironment that is optimal for healing to proceed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:27:35