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Titolo:
A new modified live equine influenza virus vaccine: phenotypic stability, restricted spread and efficacy against heterologous virus challenge
Autore:
Chambers, TM; Holland, RE; Tudor, LR; Townsend, HGG; Cook, A; Bogdan, J; Lunn, DP; Hussey, S; Whitaker-Dowling, P; Youngner, JS; Sebring, RW; Penner, SJ; Stiegler, GL;
Indirizzi:
Univ Kentucky, Dept Vet Sci, Gluck Equine Res Ctr 108, Lexington, KY 40546USA Univ Kentucky Lexington KY USA 40546 Res Ctr 108, Lexington, KY 40546USA Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 5B4, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5B4 skatoon, SK S7N 5B4, Canada Univ Wisconsin, Sch Vet Med, Dept Med Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Dept Med Sci, Madison, WI 53706 USA Univ Pittsburgh, Sch Med, Dept Mol Genet & Biochem, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 Biochem, Pittsburgh, PA 15261 USA Heska Corp, Ft Collins, CO 80525 USA Heska Corp Ft Collins CO USA 80525Heska Corp, Ft Collins, CO 80525 USA
Titolo Testata:
EQUINE VETERINARY JOURNAL
fascicolo: 7, volume: 33, anno: 2001,
pagine: 630 - 636
SICI:
0425-1644(200111)33:7<630:ANMLEI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
A VIRUSES; HORSES;
Keywords:
horse; intranasal; antigenic drift; backpassage; challenge; cold-adapted; modified-live virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Chambers, TM Univ Kentucky, Dept Vet Sci, Gluck Equine Res Ctr 108, Lexington, KY 40546USA Univ Kentucky Lexington KY USA 40546 Lexington, KY 40546USA
Citazione:
T.M. Chambers et al., "A new modified live equine influenza virus vaccine: phenotypic stability, restricted spread and efficacy against heterologous virus challenge", EQUINE V J, 33(7), 2001, pp. 630-636

Abstract

Flu Avert IN vaccine is a new, live attenuated virus vaccine for equine influenza. We tested this vaccine ill Vivo to ascertain 1) its safety and stability when subjected to serial horse to horse passage, 2) whether it spread spontaneously from horse to horse and 3) its ability to protect against heterologous equine influenza challenge viruses of epidemiological relevance. For the stability study, the vaccine was administered to 5 ponies. Nasal swabs were collected and pooled fluids administered directly to 4 successive groups of naive ponies by intranasal inoculation. Viruses isolated from the last group retained the vaccine's full attenuation phenotype, with no reversion to the wild-type virus phenotype or production of clinical influenza disease. The vaccine virus spread spontaneously to only 1 of 13 nonvaccinated horses/ponies when these were comingled with 39 vaccinates in the samefield. For the heterologous protection study, a challenge model system wasutilised in which vaccinated or naive control horses and ponies were exposed to the challenge virus by inhalation of virus-containing aerosols. Challenge viruses included influenza A/equine-2/Kentucky/98, a recent representative of the 'American' lineage of equine-2 influenza viruses; and A/equine-2/Saskatoon/90, representative of the 'Eurasian' lineage. Clinical signs among challenged animals were recorded daily using a standardised scoring protocol. With both challenge viruses, control animals reliably contracted clinical signs of influenza, whereas vaccinated animals were reliably protected from clinical disease. These results demonstrate that Flu Avert IN vaccine is safe and phenotypically stable, has low spontaneous transmissibility and is effective in protecting horses against challenge viruses representative of those in circulation worldwide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:37:02