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Titolo:
Characterization of dioxin-like activity of sediments from a Czech river basin
Autore:
Hilscherova, K; Kannan, K; Kang, YS; Holoubek, I; Machala, M; Masunaga, S; Nakanishi, J; Giesy, JP;
Indirizzi:
Masaryk Univ, Fac Sci, Dept Environm Chem & Ecotoxicol, CS-61137 Brno, Czech Republic Masaryk Univ Brno Czech Republic CS-61137 CS-61137 Brno, Czech Republic Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, E Lansing,MI 48824 USA Michigan State Univ E Lansing MI USA 48824 ol Ctr, E Lansing,MI 48824 USA Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 xicol, E Lansing, MI 48824 USA Yokohama Natl Univ, Inst Environm Sci & Technol, Hodogaya Ku, Yokohama, Kanagawa 240, Japan Yokohama Natl Univ Yokohama Kanagawa Japan 240 ohama, Kanagawa 240, Japan Vet Med Res Inst, Brno 62132, Czech Republic Vet Med Res Inst Brno CzechRepublic 62132 t, Brno 62132, Czech Republic
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
fascicolo: 12, volume: 20, anno: 2001,
pagine: 2768 - 2777
SICI:
0730-7268(200112)20:12<2768:CODAOS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HALOGENATED AROMATIC-HYDROCARBONS; TRACE ORGANIC CONTAMINANTS; TOXIC EQUIVALENT FACTORS; GENE-EXPRESSION ASSAYS; FISH HEPATOMA-CELLS; IN-VITRO BIOASSAY; AH RECEPTOR; PARTICULATE MATTER; RAINBOW-TROUT; ENVIRONMENTAL-SAMPLES;
Keywords:
in vitro bioassays; 2,3,7,8-tetrachlorodibenzo-p-dioxin; polycyclic aromatic hydrocarbons; organochlorines; dioxin-like activity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hilscherova, K Masaryk Univ, Fac Sci, Dept Environm Chem & Ecotoxicol, CS-61137 Brno, Czech Republic Masaryk Univ Brno Czech Republic CS-61137 Czech Republic
Citazione:
K. Hilscherova et al., "Characterization of dioxin-like activity of sediments from a Czech river basin", ENV TOX CH, 20(12), 2001, pp. 2768-2777

Abstract

Synthetic organic chemicals are present in environmental compartments as complex mixtures and therefore their potential effects are difficult to predict. In this study, in vitro bioassays using wild-type fish and rat hepatoma cell lines and their corresponding recombinant cell systems were used to evaluate 2,3.7,8-tetrachlorodibenzo-p-dioxin (TCDD)-like activity in extracts of sediments collected from rivers of the Czech Republic. All the sediment extracts elicited statistically significant responses in all the cell lines tested. For most sediment extracts, a complete dose-response relationship was obtained. The maximal efficacy of the samples was between 57 and 143% of the maximal induction elicited by TCDD. Greater responsiveness, sensitivity, and reproducibility were observed for recombinant than wild-type cells. Cell line-specific differences in the sensitivity to compounds present in the complex sediment extracts were observed. The TCDD equivalents (TCDD-EQs) determined from the different cell bioassays were correlated. Greater concentrations of TCDD-EQs were obtained with fish cell lines. The TCDD-EQscalculated from the results of chemical analysis of toxic equivalents (TEQs) were in good agreement with those determined by bioassays; the arty hydrocaron receptor (AhR)-effects of the identified chemicals appear to be generally additive. This indicates that most of the TCDD-like activity was accounted for by the compounds identified and quantified by instrumental analysis. Fractionation along with mass-balance calculations allowed identification of the active fractions and classes of compounds. Polycyclic aromatic hydrocarbons (PAHs) were found to be responsible for most of the AhR-mediatedactivity in sediments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:53:49