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Titolo:
The endosomal compartment is an insulin-sensitive recruitment site for GLUT4 and GLUT1 glucose transporters in cardiac myocytes
Autore:
Becker, C; Sevilla, L; Tomas, E; Palacin, M; Zorzano, A; Fischer, Y;
Indirizzi:
Solvay Pharmaceut Inc, D-30173 Hannover, Germany Solvay Pharmaceut Inc Hannover Germany D-30173 D-30173 Hannover, Germany Rhein Westfal TH Aachen, Inst Physiol, Fac Med, D-52057 Aachen, Germany Rhein Westfal TH Aachen Aachen Germany D-52057 , D-52057 Aachen, Germany Rhein Westfal TH Aachen, Interdisciplinary Ctr Clin Res BIOMAT, Fac Med, D-52057 Aachen, Germany Rhein Westfal TH Aachen Aachen Germany D-52057 , D-52057 Aachen, Germany Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 iol Mol, E-08028 Barcelona, Spain
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 12, volume: 142, anno: 2001,
pagine: 5267 - 5276
SICI:
0013-7227(200112)142:12<5267:TECIAI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEART-MUSCLE-CELLS; PHOSPHATIDYLINOSITOL 3-KINASE; SUBCELLULAR TRAFFICKING; KINETIC-ANALYSIS; ADIPOSE-CELLS; RAT HEARTS; TRANSLOCATION; MEMBRANE; CARDIOMYOCYTES; STIMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Fischer, Y Solvay Pharmaceut Inc, Hans Bockler Allee 20, D-30173 Hannover,Germany Solvay Pharmaceut Inc Hans Bockler Allee 20 Hannover Germany D-30173
Citazione:
C. Becker et al., "The endosomal compartment is an insulin-sensitive recruitment site for GLUT4 and GLUT1 glucose transporters in cardiac myocytes", ENDOCRINOL, 142(12), 2001, pp. 5267-5276

Abstract

In nonstimulated cardiomyocytes, the glucose transporter GLUT4 is confinedto intracellular vesicles forming at least two populations: a storage poolenriched in GLUT4 (pool 1) and an endosomal pool containing both GLUT4 andGLUT1 (pool 2). We have now studied the dynamics of these pools in response to insulin or the mitochondrial inhibitor rotenone in rat cardiomyocytes. Rotenone recruited GLUT4 and GLUT1 to the cell surface from endosomal pool2 without affecting pool 1. Kinetic experiments were consistent with rotenone acting on an intracellular compartment that is in close connection withthe plasma membrane. In contrast, insulin caused rapid, complete depletionof GLUT4 from pool I and reduced the GLUT1 content of pool 2 by approximately 50%, whereas, surprisingly, no net decrease in GLUT4 occurred in this pool. Subsequent insulin withdrawal resulted in slow replenishment of pool 2with GLUT1 and of pool I with GLUT4. When pool 1 was still largely depleted of GLUT4, a second insulin challenge did reduce GLUT4 in pool 2 and stimulated glucose transport to the same extent as the first insulin treatment. In conclusion, the storage pool is the primary source of GLUT4 in responseto insulin, but not to rotenone. In addition, the endosomal compartment isan important recruitment site of both GLUT1 and GLUT4 when the storage pool is either unaffected (rotenone) or depleted (by a previous insulin challenge). GLUT4 mobilized by insulin from the storage pool may pass through an intermediary (possibly endosomal) compartment on its way to the cell surface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:41:16